Detection and molecular identification of Ditylenchus destructor in goat feces
Ditylenchus destructor,a significant plant parasitic nematode,poses a severe threat to potato and sweet potato industries in China,yet its transmission mechanism remains elusive.In this study,diseased potatoes infected with D.destructor collected from fields were used to feed goats.Consequently,D.destructor eggs were detected in the goats'feces.Molecular identification confirmed that the pathogenic nematodes from the diseased potatoes belonged to haplotype B of D.destructor.Incubation and inoculation tests demonstrated that D.destructor eggs present in goat manure were capable of hatching into larvae and infecting potatoes.This study revealed for the first time that D.destructor can be transmitted through goat feces,uncovering a potential cause for sudden outbreaks of D.destructor disease in potato fields following perennial continuous corn crops in the Yulin area.These findings provide crucial theoretical support for future recommendations,including the prompt removal of diseased and rotten potatoes from fields,centralized disposal,and the strict avoidance of using un-composted goat feces as fertilizer.
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万方数据
