Description of Subanguina radicicola in Gansu province
This study aimed to clarify the species of plant parasitic nematodes on Poa pratensis in Tianzhu county,Gansu province.A combination of morphological and molecular methods was used to identify the species.The results showed that the morphometric and morphological characteristics of nematodes infecting P.pratensis were consistent with those of Subanguina radicicola(Greeff,1872)Paramonov,1967.The phylogenetic trees of rDNA-ITS and 28S rDNA D2D3 regions indicated that the population was clustered with S.radicicola population from Belgian,Yunnan,Estonian,and Russian,with high confidence.Meanwhile,laboratory pathogenicity test showed that all plants inoculated with nematodes showed similar symptoms observed in the field,and reproduction factor was 1.7.Therefore,the nematode infecting P.pratensis in Gansu was identified asS.radicicola,which is of great significance for understanding the biodiversity of Anguinidae nematodes in China and grasping the occurrence,distribution and potential risks of such nematodes.