Influence of Botrytis cinerea culture mediums and subculturing on the virulence of Bursaphelenchus xylophilus
In this study,changes in the fecundity and virulence of Bursaphelenchus xylophilus fed on Botrytis cinerea growing on eight different mediums and changes in the virulence of B.xylophilus subcultured on PDA for 84 generations were examined,using 3-year-old Pinus thunbergii as the inoculum material.The results showed that the fecundity of B.xylophilus fed on B.cinerea growing on three mediums,namely corn,wheat,and PDA,was significantly higher than those fed on B.cinerea on CDA and four synthetic mediums.The population size of B.xylophilus was highest on the combination of PDA and B.cinerea,48.94％higher than those the combination of synthetic medium C/D and B.cinerea.The disease index of P.thunbergii inoculated with B.xylophilus fed on B.cinerea on PDA was also the highest,47.87％higher than inoculation with B.xylophilus fed on B.cinerea on the synthetic medium D.However,after 14 generations of subculturing B.xylophilus fed on B.cinerea on PDA,an increase in successive generations led to a decrease in virulence.By the 79th generation,the disease index of P.thunbergii inoculated with B.xylophilus was zero.Therefore,to maintain virulence,B.xylophilus should be cultured on B.cinerea growing on PDA and subcultured for no more than 14 generations.These findings provide valuable insights into the reproduction technology and pathogenic mechanisms of B.xylophilus.
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