Control efficacy of Purpureocillium lilacinum and its compound formulations on root-knot nematode in tomato field
Root-knot nematode disease is common in agricultural production,which seriously affects the growth and yield of crops.Purpureocillium lilacinum is one of the most important biocontrol fungi,which is capable of parasitizing various important plant-parasitic nematodes such as root-knot nematodes and cyst nematodes.The colonization ability of P.lilacinum in the rhizosphere and root of tomato was investigated in facility greenhouse,and the control effects of P.lilacinum conidia,microsclerotia and its compound formulations on tomato root-knot nematodes were determined.The results showed that P.lilacinum could been detected in the rhizosphere and roots of tomato 30 d after applying three kinds of the agents,and the fungal concentrations in the roots reached 100 cfu/g.Control efficacy against tomato root-knot nematode was greater than 75％in all treatments of P.lilacinum 90 d after applying,among which the microsclerotia preparation(6 000 MS/g)had the highest efficacy of 87.27％,and the nematode density in the soil decreased by 96.0％.In addition,the P.lilacinum preparations and P.lilacinum-Clonostachys rosea multiple microbial agent(m/m=2∶1)significantly promoted plant growth and increased leaf area,while the tomato yields increased by 17.9％to 41.3％.This study provides a theoretical basis for the development and utilization of the biocontrol products of P.lilacinum,and it is of great significance to the management of plant nematodes.
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