Development and application of Bacillus subtilis ZWZ-19 dispersible oil suspension agent for Ditylenchus destructor
To develop a control agent for Ditylenchus destructor,Bacillus subtilis ZWZ-19,obtained in our previous study was used to prepare a dispersible oil suspension formulation for seed-dressing of potatoes.By testing the effects of different emulsifiers on the stability of the formulation,combining with suspension rate,pourability,particle size,static surface tension,and dynamic contact angle,the optimal formula for the ZWZ-19 dispersible oil suspension was selected:20%Bacillus subtilis mother liquor,15%NP-10,3%OMMT,0.5%ethylene glycol,3%white carbon black,and soybean oil to make up 100%.The suspension rate of the formulation was above 95%before and after thermal storage,and the decomposition rate of the active ingredient was less than 5%.Field test results showed that the ZWZ-19 dispersible oil suspension had a control efficacy of 69.61%against stem rot nematode disease caused by Ditylenchus destructor,with a yield increase effect of 16.38%.