The malectin-like kinase MdFER1 negatively regulates resistance to Valsa canker
Screening for resistance genes can provide valuable genetic materials for breeding apple varieties with enhanced resistance.We analyzed the role of MdFER1,a member of the Catharanthus roseus receptor-like kinase-1-like(CrRLK1L)proteins subfamily,in apple Valsa canker resistance through bioinformatics analysis,expression analysis,and functional verification of the gene.The results showed that MdFER1 is homologous to Arabidopsis FER,although the sequence similarity is only 46.4％.Transient expression of MdFER1 in either'Yanfu 6'fruits or'Wanglin'suspension cells significantly reduced resistance to apple Valsa canker.Additionally,MdFER1 transient expression significantly activated the expression of genes associated with reactive oxygen species(TaNOX)and hypersensitive response(HSR 203),but inhibited the expression of defense-related genes PR1 and EDS1.In summary,MdFER1 negatively regulates resistance to apple Valsa canker in'Yanfu 6'fruits or'Wanglin'suspension cells.The activation of ROS and HR signaling,along with the inhibition of defense-related gene expression,are key factors contributing to the negative regulation of apple Valsa canker resistance by MdFER 1.
apple Valsa cankerCatharanthus roseus receptor-like kinase-1-likemarker gene
NETL
NSTL
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