DNA barcoding identification of Aegilops tauschii and Ae.cylindrica in wheat fields
Aegilops tauschii Coss.and Ae.cylindrica Host.,both malignant weeds in wheat fields,are considered quarantine pests in China.The morphological similarity between Ae.cylindrica and Ae.tauschii complicates targeted weed management in agricultural production.To address this,this study utilized DNA barcoding technology to differentiate and identify these weeds.A total of 52 samples from 9 provinces,including Hebei,Henan,and Shandong,were analyzed.Four DNA barcode sequences(rbcL,matK,trnH-psbA,andITS2)were sequenced.Intraspecific and interspecific genetic distances were calculated,and a phylogenetic tree was constructed using the neighbor-joining method.The results showed that among the four DNA barcodes,matK sequencing was unsatisfactory.The rbcL sequences of the two species were identical.The intraspecific genetic distance of ITS2 was 0.000 7,while its interspecific genetic distance was 0.009 6.The trnH-psbA sequence accurately identified Ae.tauschii and Ae.cylindrica,showing 15 different interspecific loci with a genetic distance of 0.005 1,and no intraspecific differences.The neighbor-joining tree had a frequency of 96.0％.In specificity testing,both Ae.tauschii and Ae.cylindrica exhibited specific bases,confirming trnH-psbA as the optimal identification sequence.This study provides a novel molecular approach for the rapid and accurate identification of these two quarantine weeds.
NETL
NSTL
万方数据
