Evaluation of resistance to Fusarium head blight and analysis of DON toxin content in kernel among 55 wheat cultivars and reserve varieties
In order to explore resistant germplasm resources and resistant genes to Fusarium head blight(FHB)and evaluate the relation between DON toxin content in kernel and resistant level in cultivar,55 wheat cultivars and reserve varieties were identified for resistance to FHB by double florets injection in 2021 and screened using molecular markers related to Fhb1,Fhb2,Fhb4,Fhb5 and QFhs.crc-2DL,and also their DON toxin content in kernel were determined.'Sumai 3''Zhengmai 9023''Xiaoyan 22'and'Huamai 1169'were used as the control of highly resistant,moderately resistant,moderately susceptible and highly susceptible to FHB,respectively.The results showed that 21 cultivars were moderately resistant to FHB.Among the tested 55 cultivars,only'Huamai169'carried Fhb1 with the average diseased spikelets rate of 22.34％,5 cultivars carried Fhb2 with the average diseased spikelets rate from 28.34％to 45.92％,5 cultivars carried Fhb4 with the average diseased spikelets rate from 26.73％to 65.59％,13 cultivars carried QFhs.crc-2DL with the average diseased spikelets rate from 32.96％to 70.81％,while Fhb5 were not detected in 55 cultivars.Among the 21 moderately resistant cultivars,no known resistant genes were detected in 16 cultivars,presuming that unknown resistant genes were contained in these cultivars.The results of DON toxin content detection showed that in 21 moderately resistant cultivars,the highest DON content in some cultivars was more than eight times than the lowest DON content in some cultivars,DON content in eight cultivars were more than 3 500 μg/kg.Among the 13 moderately susceptible cultivars,the highest DON content in some cultivars was more than four times than the lowest DON content in some cultivars,DON content in'Yangmai 13''Emai 352'and'Jingmai 66'were less than 3 500 μg/kg,while DON content in all the other 10 cultivars were more than 3 500 μg/kg.The above results illustrated that the resistance identification information in the field was deemed insufficient for identifying valuable germplasm resources used for genetic improvement of resistance in Fusarium head blight.It is necessary for kernel DON toxin content measurement even if the cultivar was identified as moderately resistant to FHB,which will contribute to comprehensively evaluate the resistance potential of these cultivars.
wheatFusarium head blightresistancemolecular markerDON contentgermplasm
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NSTL
万方数据
