Effects of nonprotein toxin from Arthrinium phaeospermum on physiological metabolism of Bambusa pervariabilis × Dendrocalamopisis grandis hybrid bamboo
According to the nonprotein toxin isolated by silica gel column chromatography, bioassay was tested by impregnation method in hybrid bamboo. Moreover, effects of different concentrations of toxin on the relative permeability of cell membrane, the contents of soluble protein, soluble sugar, malondialdehyde (MDA), free proline (Pro) and the activities of superoxide dismutase (SOD), peroxidase (POD), pheny-lalanine amonnialyase (PAL) were analyzed by the leaf disk method. The results showed that the typical symptoms were indicated in the toxin treatments. The relative permeability of cell membrane and the content of MDA were significantly increased. When treated with the low concentrations of toxin, there was a positive correlation between the content of free Pro, the activities of enzymes and toxin. The correlation between the content of soluble sugar and the concentration of toxin was negative. However, when treated with the high concentrations of toxin, there was a negative correlation between the content of free Pro, the activities of enzymes and toxin. This demonstrated that high concentrations of toxin could destroy the tissues of hybrid bamboo and toxin played an important role in pathogenicity of A. Phaeospermun.
pathogenic toxinphysiology and biochemistryresistance associated enzyme
NETL
NSTL
万方数据
维普
