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欧李褐腐病病原菌鉴定

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Identification of the pathogen causing brown rot of Chinese Dwarf Cherry( Cerasus humilis
One serious disease happened on the fruit of Chinese Dwarf Cherry in Liaoning Province recently. The typical symptom was brown spot formed on the fruit surface. The spot spread quickly through the whole fruits, then the fruits were rotten. There were tomentum round shaped mildew formed on the surface of symptomatic fruits. The pathogen was isolated from infected fruits. After pathogenicity tests in lab and field and re-isolation, the isolate HI was determined to be responsible for the disease. HI colony on PDA was 70 -75 mm in diameter, with concentric rings in grayish or light brown color after 7 days incubation at 221 with illumination of 12 h near-UV/12 h dark. The conidia were one-celled, hyaline, lemon-shaped, (10-27) jun x (7-17) jun on PDA, produced in branched monilioid chains. The rDNA ITS sequence of islolates HI and Gl had 100% similarity with those of Monilinia fructicola in GenBank. The amplification results with three Monilinia specific primer pairs showed that only primer pair ITSlMfcl and ITS4Mfcl could amplify a 356 bp fragment. Based on the morphological characteristics and rDNA molecular analysis, the pathogen was finally identified as M. Fructicola.

Chinese Dwarf CherryMonilinia fructicolamorphological identificationITS sequence analysisspecific amplification

徐成楠、周宗山、吴玉星、迟福梅、张红军

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中国农业科学院果树研究所,兴城125100

Chinese Dwarf Cherry Monilinia fructicola morphological identification ITS sequence analysis specific amplification

2011

植物病理学报
中国植物病理学会

植物病理学报

CSTPCDCSCD北大核心
影响因子：0.865
ISSN：0412-0914
年,卷(期)：2011.41(6)
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