Molecular identification of a phytoplasma associated with cherry virescence disease
Using nested PCR with phytoplasma universal primer pair for 16S rDNA and Elm yellows (EY) group-specific primer pair for ribosomal protein gene operon to detect phytoplasma associated with cherry virescence (ChV) disease in Mianning County, Sichuan Province. DNA fragments about 1.2 kb were amplified from the total DNA of diseased cherry samples, respectively. After cloning, necleotide sequencing and searching similar sequences of BLAST in GenBank, construction of phylogenetic tree and online analysis of iPhyClassifier for 16S rDNA were made. The results indicated that the phytoplasma ChV was related to ' Candidates Phytoplasma ziziphi' and belonged as variant of subgroup 16SrV-B. The comparative sequece and phylogenetic analyses of ribosomal protein gene revealed that the ChV phytoplasma represented a new rp gene lineage, designated as rpV-N. Phylogenetic analyses of rp gene sequences allowed clearer distinctions between ChV and closely related strains than did analysis of 16S rDNA.
NETL
NSTL
万方数据
维普
