首页|小麦白粉菌HSP70基因的克隆及高温胁迫对其表达的影响

小麦白粉菌HSP70基因的克隆及高温胁迫对其表达的影响

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Cloning of heat shock protein gene, HSP70, in Blumeria graminis f.sp.tritici and its expression under high temperature stress
The objective of this study was to detect the effect of high temperature stress on the expression of HSP70 gene in Blumeria graminis f. sp. tritici. The cDNA sequence of HSP70 gene was cloned using RT-PCR and RACE. The obtained cDNA sequence contained a 1 947 bp open reading frame (ORF) and encoded a sequence of 648 amino acids residues. The molecular mass was estimated as 70. 5 kDa and isoelectric point (pI) 4. 84. The gene contained the signature sequences of HSP70 family. Real-time quantitative PCR was used to analyze the expression of HSP70 gene of B. graminis f. sp. tritici after the treatments of heat shock (28℃) in different time. The results showed that the mRNA expression of HSP70 gene of B. graminis f. sp. tritici was significantly up-regulated after heat shock at 28℃. The expression level of HSP70 gene reached the maximum (11.6 times of the control) after 60 minutes, and then decreased. It was inferred that HSP70 gene might play an important role in B. graminis f. sp. tritici to high temperature.

Blumeria graminis f. sp. triticiHSP70gene clonehigh temperature stressreal-time quantitative PCR

邢淑莲、周益林、段霞瑜、邹亚飞

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中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

Blumeria graminis f. sp. tritici HSP70 gene clone high temperature stress real-time quantitative PCR

国家重点基础研究发展规划(973计划)国家重点基础研究发展规划(973计划)国家自然科学基金农业部财政专项

2010CB9515032013CB127700312719872130108

2013

植物病理学报
中国植物病理学会

植物病理学报

CSTPCDCSCD北大核心
影响因子：0.865
ISSN：0412-0914
年,卷(期)：2013.43(2)
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