Cloning and expression of mitogen-activated protein kinase homolog gene UeKss1in Ustilago esculenta
The dimorphism of the endophytic fungus Ustilago esculenta may be closely related to the stem swollen of Zizania latifolia.MAPK (mitogen activated protein kinase) signaling pathway has been known to play important role of regulation in the fungi dimorphism.In this study,we cloned the gene UeKss1 encoding a MAPK protein.Clustering analysis showed that UeKss1 with high conserved Serine/threonin dual specific protein kinase catalysis domain,shared more than 80％ homology with other fungi in Ustilago.Growth characteristics of U.esculenta and expression analysis of UeKss1 under induction of different carbon resources showed that fungi hyphae formation was only induced by sucrose,with up-regulated expression of UeKss1.While yeast forms were found in PDA,glucose and maltose media.The expression of UeKss1 had no significant changes when induced by PDA and glucose.In contrast,it was obviously up-regulated when induced by maltose even more than sucrose.In addition,UeKss1 was successfully expressed and purified in prokaryotic expression systems and confirmed by western blot.All the work provided the basic materials for further functional study of UeKss1 and exploring of the mechanism on dimorphism regulation in U.esculenta.
Ustilago esculentaUeKss1mitogen-activated protein kinasedimorphism
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