Endophytic fungi have great potential in biological control,and the objective of this study is to explore antagonistic action of endophytic fungus Chaetomium globosum CEF-082 against Verticillium dahliae and its control effect against Verticillium wilt of cotton.The inhibition rate of CEF-082 aganist V.dahliae was assessed using the exudate filtering method and concave slide method,and the control effect of C.globosum on cotton Verticillium wilt were detected by culture filtrate (CF) inoculation and substrate inoculation methods in greenhouse.In addition,the expression level of defense genes and DNA content of V.dahliae in Gossypium hirsutum were detected by real-time quantitative PCR.The results showed that the inhibition rate of non-volatile metabolites of CEF-082 on the colony growth of V.dahliae was 100％,and on conidial yield was 70.5％.However,obvious inhibitory effect of CEF-082 on conidial germination was not detected.When cotton seedlings were pro-inoculated with CEF-082 CF or substrate containing CEF-082 in greenhouse,the inhibition rates were 62.37％ and 66.88％,respectively.These two treatments had no negative effect on the growth of cotton,and the latter treatment promoted seedling emergence.In addition,fluorescence quantitative PCR results showed that CEF-082 metabolic products significantly increased the expression of cotton defense genes including pod,ppo,pal and β-1,3-glucanase,and inhibited the multiplication of V.dahliae in G.hirsutum.Endophytic fungus CEF-082 in G.hirsutum can effectively control Verticillium wilt,and its action mechanisms include anfibiotc effect and induced resistance of host plant.Taken together,our findings suggest that endophytic fungus CEF-082 has great potential in controlling cotton Verticillium wilt.
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