Ultrastructural observation of infection process of Phoma bellidis causing stem blight of Eleocharis dulcis
The present work demonstrates infection process of Phoma bellidis on Eleocharis dulcis.Conidial suspensions of the fungus were inoculated onto healthy stems of resistant cultivar Xiangtan and susceptible cultivar Lushan,respectively,and the infection process was observed by scanning electron microscopy.Conidia started to germinate with one or more germ tubes at 6 hours post inoculation (hpi).At 12 hpi,the germ tubes penetrated into epidermal cells directly,penetrated cuticle by forming a cushion-shaped appressorium or entered into cell through the opened stomata.Direct penetration was the most frequent style for P.bellidis infection.The pene trated hyphae could also form branches for further infection of host cells.At 24 hpi,no significant difference in conidial germination rate was found between resistant and susceptible cultivars,while significant differences were observed in both length of germ tube and infection rate.Lower penetration rate might be an important factor for resistance of cultivar Xiangtan which caused unsuccessful penetration of germ tube to grow continuously on the surface of host cells.This is the first report of the infection process of Phoma bellidis on host plant.
Eleocharis dulcisinfectionPhoma bellidisscanning electron microscopy
赵玳琳、陈威、黄俊斌、刘浩、陶刚、郑露
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华中农业大学,湖北省作物病害监测与安全控制重点实验室,武汉430070
贵州省植物保护研究所,贵阳550006
Eleocharis dulcis infection Phoma bellidis scanning electron microscopy
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