Effect of acetic acid on growth and development of Purpureocillium lilacinum and its nematocidal activity
To investigate the effect of acetic acid on the growth development and nematocidal activity of Purpureocillium lilacinum,the growth rate,spore germination and production,and colony morphology of P.lilacinum were assayed in the presence of acetic acid with different concentrations.The growth development of P.lilacinum was not impaired in the culture medium containing low-dosage acetic acid (200 μg · mL-1).P.lilacinum could produce acetic acid with the concentration up to 146 μg · mL-1 in PDB medium at 3 days post-inoculation,whereas the concentration of it was decreased when the carbon sources were gradually consumed in PDB medium.It was observed that limited carbon sources of culture medium resulted in none of acetic acid produced by P.lilcacinum.In addition,P.lilacinum exhibited good growing status in the medium containing acetate with concentrations ranging from 3 mg · mL-1 to 10 mg · mL-1,which suggested that P.lilacinum could make use of acetic acid radical as its carbon source.Furthermore,acetic acid could enhance P.lilacinum nematocidal activity,and the concentration of acetic acid was positive correlation to the inhibition of egg hatch and lethal effect on second-stage juvenile (J2) of root-knot nematode caused by P.lilacinum.
NETL
NSTL
万方数据
维普
