Fungal community diversity in citrus fruit at different ripening stage
Postharvest citrus fruit decay,caused by fungi,is a common disease which has great impact on citrus industry.There are diverse fungal communities in citrus fruit under natural conditions.By decoding the sequences of fungal ITS region through Illumina MiSeq sequencing technologies,we analyzed the diversity and dynamic of fungal communities in the rind and flesh of Wanmi No 1 citrus fruit samples at the growth,ripening and storage stages.Through analyses of OTU with abundance,diversity index,and community structure at the order and genus level,we found that there was a higher degree of fungal diversity in the rind than in the flesh at both the growth and storage stage,on the contrary,the opposite results were found at the ripening stage of citrus fruit.The dominant genera were different at different stage,with Medicopsis and Collectotrichum in the rind at the growth stage,Collectotrichum at the ripening stage,Botrytis,Erythrobasidium and Strelitziana at the storage stage;while in the flesh,Penicillium and Cladosporium accounted for 1/4 of the whole community at the growth stage,Botrytis with more than 50％ of the total at the ripening stage,Penicillium and Alternaria accounted for 90％ in which 2/3 was Alternaria at the storage stage.The population of plant pathogenic fungi Cladosporium,Magnaporthe,Sclerotinia,Botrytis,Erysiphe,Penicillium,Altemaria and Fusarium in the rind were larger than in the flesh.The large population of fungi and the change pattern in the flesh suggest that the postharvest fruit decay should be a result of interaction of fungi.The results will help us to understand the disease cycles and design control techniques of citrus postharvest fruit decay in advance to flowering stage.
citrusfungal community diversityIllumina MiSeqpostharvest fruit decay
NETL
NSTL
万方数据
维普
