金森女贞叶斑病病原物的鉴定、生物学特性及防治药剂筛选
Identification,characterization and fungicide screening of the pathogen causing leaf spot on Ligustrum japonicum
陈宸 1沙鹏飞 2江学 2江珊 2杨峰 2沈迎春 3陈夕军1
作者信息
- 1. 扬州大学植物保护学院,江苏扬州 225009;教育部农业与农产品安全国际合作联合实验室,江苏扬州 225009
- 2. 扬州大学植物保护学院,江苏扬州 225009
- 3. 江苏省农药总站,江苏南京 210036
- 折叠
摘要
金森女贞叶斑病每年春季在扬州大学文汇路校区均普遍发生.为明确其病原种类及病菌生物学特性,筛选适于防治该病的化学药剂,作者对病原菌进行分离纯化、室内外离体与活体致病性测定、形态与多基因(ITS、GAPDH、RPB2和TEF1)序列分子鉴定,以及病原菌生物学特性和对5种杀菌剂的敏感性测定.结果表明,金森女贞叶斑病病原物为链格孢(Alternaria alternata),其最适生长条件为:以蔗糖或果糖为碳源、以硝酸钾为氮源,在25 ℃、pH 9.0条件下生长.5种杀菌剂对A.alternata均表现较强的毒力,其中嘧菌酯毒力最强,EC50值为0.080 6 μg·mL-1;咪鲜胺、吡唑醚菌酯和戊唑醇次之,EC50 值分别为2.272 2 μg·mL-1、3.934 9 μg·mL-1 和6.400 0 μg·mL-1;而苯醚甲环唑的毒力最弱,EC50 值为 15.486 0 μg·mL-1,说明这些药剂均可用于生产上金森女贞叶斑病的防控.
Abstract
Ligustrum japonicum leaf spot occurs universally in Wenhuilu campus of Yangzhou university,Yan-gzhou,Jiangsu,China every spring.The aim of this study was to identify the pathogen species,determine the biological characteristics,and screen out effective pesticides for the prevention and control of the disease.The multiple assays were conducted for the pathogen identification including tissue separation and single-spore purifi-cation,pathogenicity test both in vitro and vivo to fulfill Koch's postulates,and morphological and phylogenetic analysis based on a combined ITS,GAPDH,RPB2 and TEF1 sequence dataset,the biological characterization,and the sensitivity test to the five fungicides.We obtained the purified fungal culture which was further identified as Alternaria alternata,a new pathogen causing leaf spot on L.japonicum.The effect of various carbon and ni-trogen sources for growing of the representative isolate was investigated and A.alternata isolate was suitable for growing on the optimized medium supplied with sucrose,fructose,and potassium nitrate at 25 ℃,pH 9.0.In addition,5 fungicides were assessed on the inhibitory effect against mycelial growth of A.alternata isolate in which azoxystrobin displayed the strongest inhibitory activity with EC50 value of 0.080 6 μg·mL-1,followed by-prochloraz,pyraclostrobin and tebuconazole with EC50 values of 2.272 2 μg·mL-1,3.934 9 μg·mL-1 and 6.400 0μg·mL-1,respectively,while difenoconazole exhibited the least sensitivity at EC50 value of 15.486 0 μg·mL-1.These results indicated that these fungicides could be used for the prevention and control of L.japonicum leaf spot disease.
关键词
病原鉴定/生物学特性/药剂筛选/链格孢/金森女贞叶斑病Key words
identification of pathogen/biological characteristics/fungicide screening/Alternaria alternata/Ligustrum japonicum leaf spot引用本文复制引用
基金项目
江苏省现代农业产业技术体系建设项目(JATS2021338)
扬州大学大学生科技创新项目(X20220622)
出版年
2024
NETL
NSTL
万方数据