Screening and identification of effector candidates in the late stage of interaction between Alternaria solani and potato
The potato early blight caused by Alternaria solani is an important disease in potato production,which seriously affects the yield and quality of potatoes.To reveal the interaction mechanism between A.solani and its host plant potato,we screened the effector candidates produced by A.solani during its interaction with potato plant using RNA transcriptome sequencing technology and biological software(signalp-4.1,tmhmm-2.0c,phobius101 and ProtComp v3)combined with characteristics analysis of effector proteins.As a result,137 differentially expressed candidate proteins were obtained,and four(Gene03396,Gene06670,Gene02858 and Gene06508)of them were selected for further analysis.Based on the result of RNA-seq analysis,Gene03396,Gene06670 and Gene02858 had an up-regulated expression pattern at three time points(3,4 and 5 dpi)com-pared with that at 0 dpi,whereas Gene06508 had a downregulated expression pattern.The four proteins do not have a common motif according to the MEME analysis result.Transient overexpression of the target effector candidate genes in tobacco showed that Gene03396 and Gene02858 directly induced tobacco cell necrosis,and Gene06670 inhibited programmed cell death(PCD)induced by BCL2-associated X,indicating that effector candidates function by stimulating plant immunity or inhibiting host defense response.The subcellular localiza-tion results revealed that Gene03396 was located on the cell membrane,while Gene06670 and Gene02858 may be located in the intercellular space.These results provide a basis for further identifying the effector proteins of A.solani and revealing the underlying pathogenic mechanism of the pathogen.
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