Histological observation and the defense-related gene expression pattern analysis of wheat nonhost resistance to poplar leaf rust
Poplar leaf rust is serious harm to the tree health and planting resistance cultivar is an effective way to control the disease.The breakdown of resistance owing to the variation of pathogen,it is urgent for exploring new resistance resources.Plant nonhost resistance,characterized by broad-spectrum and durability,provides a new resolution for resistance breeding.In the present study,the histology observation and the expression pattern of defense-related genes of wheat leaves inoculated with Melampsora larici-populina(Mlp),the casual agent of popular leaf rust,were analyzed.The results demonstrated that although Mlp urediniospores normally germinated on wheat leaves,only 3.7% recognized the stomata,and a very few of them were able to develop sub-stomatal vesicle-like(SSVL)structure and infection hypha.The immediately induction of H2O2 and callose at the infec-tion site arrested the further infection of the rust fungi.In addition,no cell death was observed,suggested that the defense reaction of wheat against Mlp belongs to type Ⅰ nonhost resistance.Two genes involved in jasmonic acid synthesis,LOX1 and AOS1,as well as PRla,the downstream pathogenesis related gene of JA pathway,were all induced by Mlp infection,highlighted an important role of JA pathway in wheat nonhost resistance to Mlp.This study provided groundwork for further elucidation of the molecular mechanism of wheat nonhost resis-tance to poplar leaf rust.
wheatnonhost resistancepoplar leaf rustjasmonic acidreactive oxygen species
NETL
NSTL
万方数据
