基于转录组分析的禾谷镰孢菌对SDHIs杀菌剂的胁迫响应机制
Stress response mechanism of Fusarium graminearum to SDHIs based on transcrip-tome analysis
李新蕾 1朱孟坤 2李伟 2曹淑琳 2邓清超 3方守国 3陈怀谷 2孙海燕2
作者信息
- 1. 长江大学农学院,荆州 434025;江苏省农业科学院植物保护研究所,南京 210014
- 2. 江苏省农业科学院植物保护研究所,南京 210014
- 3. 长江大学农学院,荆州 434025
- 折叠
摘要
在植物病害化学防治研究中,杀菌剂的作用机制和病原菌的抗药性机制是关注的重点,而病原菌如何响应并减轻化学药剂胁迫的机制往往被忽略.本研究利用转录组测序技术研究了禾谷镰孢菌(Fusarium graminearum)对琥珀酸脱氢酶抑制剂类(SDHIs)杀菌剂的胁迫响应机制.分析结果表明:与对照样品相比,氟唑菌酰羟胺和苯并烯氟菌唑处理后病原菌共同上调表达的差异基因有1180个,共同下调表达的差异基因有937个.已知SDHIs杀菌剂作用于病原菌线粒体电子传递链复合物Ⅱ,本研究重点分析了与线粒体电子传递链相关基因的表达,发现F.graminearum通过上调表达琥珀酸脱氢酶、泛醇-细胞色素C还原酶、延胡索酰乙酰乙酸水解酶(FAH)、ABC、MFS转运蛋白等基因,下调表达SWI/SNF复合物等基因来响应SDHIs杀菌剂的胁迫.其中,FgFAH基因在SDHIs杀菌剂胁迫后被诱导表达,其敲除突变体对SDHIs杀菌剂的敏感性增强,表明该基因介导了 F.graminearum对SDHIs杀菌剂胁迫的响应调控.研究结果将为研究病原菌对化学药剂胁迫的响应机制奠定基础,并为将来开发杀菌剂的协同增效药剂提供研究思路.
Abstract
In the researches of chemical control of plant diseases,the mechanism of action of fungicides and pathogen resistance to fungicides are the focus of attention,while the mechanisms of how pathogenic bacteria re-sponse to mitigate chemical stress is often ignored.The transcriptome sequencing technology was used to study the stress response mechanism of Fusarium graminearum to succinate dehydrogenase inhibitor(SDHIs)fungi-cides in this study.The results showed that 1 180 up-regulated and 937 down-regulated differentially expressed genes were identified in the pathogen treated with pydiflumetofen and benzovindiflupyr compared to the control.Complex Ⅱ on mitochondrial electron transfer chain is the target of SDHIs fungicides.Therefore,the genes rela-ted to mitochondrial electron transfer chain were focused in this study.The results showed that F.graminearum responded to the stress of SDHIs fungicides by upregulating the expression of genes such as Succinate dehydro-genase,Ubiquinol-cytochrome C reductase,Fumarylacetoacetate hydrolase(FAH),ABC and MFS transpor-ters,and downregulating the expression of genes in the SWI/SNF complex and other genes.FgFAH gene was induced to express after being treated with SDHIs fungicides.The sensitivity of FgFAH gene-knockout mutant to SDHIs fungicide was increased compared to wild type,indicating that this gene may mediated the stress response of F.graminearum to SDHIs fungicide.The results of this study will provide a research basis for studying stress response mechanism of pathogen to chemical agents and research ideas for developing sensitizers of fungicides in the future.
关键词
禾谷镰孢菌/琥珀酸脱氢酶杀菌剂/线粒体电子传递链/转录组分析Key words
Fusarium graminearum/succinate dehydrogenase inhibitor/mitochondrial electron transfer chain/transcriptome analysis引用本文复制引用
基金项目
江苏省农业科技自主创新项目(CX212037)
现代农业产业技术体系建设项目(CARS-3)
&&(2022YFD1400105)
出版年
2024
NETL
NSTL
万方数据