Stress response mechanism of Fusarium graminearum to SDHIs based on transcrip-tome analysis
In the researches of chemical control of plant diseases,the mechanism of action of fungicides and pathogen resistance to fungicides are the focus of attention,while the mechanisms of how pathogenic bacteria re-sponse to mitigate chemical stress is often ignored.The transcriptome sequencing technology was used to study the stress response mechanism of Fusarium graminearum to succinate dehydrogenase inhibitor(SDHIs)fungi-cides in this study.The results showed that 1 180 up-regulated and 937 down-regulated differentially expressed genes were identified in the pathogen treated with pydiflumetofen and benzovindiflupyr compared to the control.Complex Ⅱ on mitochondrial electron transfer chain is the target of SDHIs fungicides.Therefore,the genes rela-ted to mitochondrial electron transfer chain were focused in this study.The results showed that F.graminearum responded to the stress of SDHIs fungicides by upregulating the expression of genes such as Succinate dehydro-genase,Ubiquinol-cytochrome C reductase,Fumarylacetoacetate hydrolase(FAH),ABC and MFS transpor-ters,and downregulating the expression of genes in the SWI/SNF complex and other genes.FgFAH gene was induced to express after being treated with SDHIs fungicides.The sensitivity of FgFAH gene-knockout mutant to SDHIs fungicide was increased compared to wild type,indicating that this gene may mediated the stress response of F.graminearum to SDHIs fungicide.The results of this study will provide a research basis for studying stress response mechanism of pathogen to chemical agents and research ideas for developing sensitizers of fungicides in the future.
Fusarium graminearumsuccinate dehydrogenase inhibitormitochondrial electron transfer chaintranscriptome analysis
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