Study on virus content of wheat yellow mosaic disease at different disease grade
In order to clarify the variation of virus content of wheat yellow mosaic virus(WYMV)in various disease grade and tissue parts,wheat samples with typical incidence levels of 0,1,2 and 3 were collected from fields with uniform incidence of wheat yellow mosaic disease.Healthy wheat samples from virus-free fields were used as blank control.The absolute quantification of the content of wheat yellow mosaic virus in roots,stems and leaves was carried out by real-time fluorescent quantitative PCR(RT-qPCR).The results showed that the content of WYMV in wheat roots was significantly higher than that in leaves(P＜0.05),and the virus content in wheat roots of grade 0 was 13.97 times higher than that in leaves.In wheat tissues of different disease grade,the virus content in roots,stems and leaves increased significantly with the aggravation of disease grade(P＜0.05).The virus content in roots increased rapidly and then slowed down.From grade 0 to grade 1,the virus content in roots increased by 3.95×106 copies·μL-1.However,from grade 1 to grade 3,the virus increase was only 1.45×106 copies·μL-1.The virus content in stems and leaves showed a trend of uniform increase,and there was a strong positive correlation between the virus content in stems and the disease grade(R2=0.996 7).This study confirmed that the root is the key tissue site of WYMV virus infection in wheat.At the same time,it was found that the content of WYMV in the roots of diseased grade 0 wheat was much higher than that in the con-trol group.The content of WYMV in the stem correlates closely with viral symptoms.The higher the content of stem virus,the more obvious the symptom of wheat.
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万方数据
