摘要
为明确小麦黄花叶病毒(wheat yellow mosaic virus,WYMV)在不同发病级别、不同组织部位含量的差异,从小麦黄花叶病发病的田块,选取发病典型的级别为0、1、2和3级的小麦样本;取无病毒田块的健康小麦样本为空白对照,利用实时荧光定量PCR(real-time fluorescent quantitative PCR,RT-qPCR)技术,分别对小麦的根、茎、叶进行小麦黄花叶病毒含量的绝对定量.结果表明,在感病样品中,小麦根部的WYMV含量都显著多于叶部(P＜0.05),发病0级小麦根部的病毒含量是叶部病毒含量的13.97倍.在不同发病级别的小麦组织中,随着发病级别的加重,根、茎、叶中的病毒含量都是显著增加的(P＜0.05).根中的病毒含量先是快速增加而后缓慢增加,0级到1级,根中病毒量增加3.95×106 copies·μL-1,而1级到3级,病毒增加量仅为1.45×106 copies·μL-1;茎和叶中病毒含量呈现匀速增加的趋势,且茎中病毒含量与发病级别呈强正相关性(R2=0.996 7).本研究明确了感病小麦根部WYMV的含量最多,同时发现在尚未显症的发病0级小麦中,根部WYMV含量远大于对照组;茎部WYMV的含量与症状关系最为密切,茎部病毒含量越高,小麦显症越明显.
Abstract
In order to clarify the variation of virus content of wheat yellow mosaic virus(WYMV)in various disease grade and tissue parts,wheat samples with typical incidence levels of 0,1,2 and 3 were collected from fields with uniform incidence of wheat yellow mosaic disease.Healthy wheat samples from virus-free fields were used as blank control.The absolute quantification of the content of wheat yellow mosaic virus in roots,stems and leaves was carried out by real-time fluorescent quantitative PCR(RT-qPCR).The results showed that the content of WYMV in wheat roots was significantly higher than that in leaves(P＜0.05),and the virus content in wheat roots of grade 0 was 13.97 times higher than that in leaves.In wheat tissues of different disease grade,the virus content in roots,stems and leaves increased significantly with the aggravation of disease grade(P＜0.05).The virus content in roots increased rapidly and then slowed down.From grade 0 to grade 1,the virus content in roots increased by 3.95×106 copies·μL-1.However,from grade 1 to grade 3,the virus increase was only 1.45×106 copies·μL-1.The virus content in stems and leaves showed a trend of uniform increase,and there was a strong positive correlation between the virus content in stems and the disease grade(R2=0.996 7).This study confirmed that the root is the key tissue site of WYMV virus infection in wheat.At the same time,it was found that the content of WYMV in the roots of diseased grade 0 wheat was much higher than that in the con-trol group.The content of WYMV in the stem correlates closely with viral symptoms.The higher the content of stem virus,the more obvious the symptom of wheat.
基金项目
国家小麦产业技术体系建设项目(CARS-03)
国家科技支撑计划(2015BAD26B01)
河南省现代农业产业技术体系建设项目漯河综合试验站项目(HARS-22-01-Z4)
NETL
NSTL
万方数据