Isolation and identification of pathogen causing leaf spot of Dysosma versipellis in Shiyan of Hubei Province
In May of 2021,a leaf spot disease of the herbal medicine Dysosma versipellis was observed,and it occured seriously in Shiyan of Hubei Province.Diseased leaves of D.versipellis were collected,pathogen was isolated by tissue cultural-method,and its pathogenicity was confirmed according to the Koch's postulates.Based on the morphological characteristics and phylogenetic analysis by combination of ITS region and EF1-α gene sequences,pathogen was identified as Alternaria alternata.This is the first report leaf spot of Dysosma versipel-lis caused by A.alternata in China.
Dysosma versipellisleaf spotAlternaria alternataHubei province
蔡高磊、周炀、张泽志、宋伟、张含、孙良学、柯尊伟、刘新刚
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丹江口库区十堰生态农业研究院,十堰 442000
十堰市龙王寨珍稀中草药种养农民专业合作社,十堰 442000
汉江师范学院化学与环境工程学院,十堰 442000
中国农业科学院植物保护研究所,北京 100193
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Dysosma versipellis leaf spot Alternaria alternata Hubei province