快速高效鉴定花生青枯病抗性的水培接种新方法研究
Development of a water culture inoculation method for quick identification of bacte-rial wilt resistance in peanut
宋万朵 1晏立英 1于东洋 1康彦平 1雷永 1陈玉宁 1淮东欣 1王欣 1王志慧 1罗怀勇 1姜慧芳 1廖伯寿1
作者信息
- 1. 油料作物生物学与遗传育种重点实验室/中国农业科学院油料作物研究所,武汉 430062,中国
- 折叠
Abstract
In order to improve the efficiency and reliability of bacterial wilt resistance screening in peanut,a novel water culture inoculation system was developed.Resistant peanut variety'Zhonghua21'and susceptible'Zhonghua12'were inoculated with mixed suspension of three Ralstonia solanacearum isolates under(28±2)℃,and 16 h light/8 h dark conditions.The minimum concentration for distinguishing the resistant and suscepti-ble peanut lines was 1×107 CFU·mL-1.The optimal time for scoring the resistance was 12 d after inoculation.36 peanut accessions were inoculated by the mixed bacterial suspension in water culture,in which 25 ones were re-sistant and 11 ones were susceptible.The same set of peanut accessions was also tested for their resistance to bac-terial wilt in natural disease nursery,from which 27 ones were resistant and nine were susceptible.The consisten-cy of these accessions for resistance to bacterial wilt both in water culture and in natural disease nursery was more than 80% .Therefore,the established method was quick,simple and high throughput for identifying resist-ance to bacterial wilt in peanut.
关键词
Peanut/bacterial wilt/resistance identification/water culture inoculation systemKey words
Peanut/bacterial wilt/resistance identification/water culture inoculation system引用本文复制引用
基金项目
中央级公益性科研院所基本科研业务费专项(1610172021003)
现代农业产业技术体系建设项目(CARS-13)
国家自然科学基金(32161143006)
湖北省重点研发计划(2021BBA077)
出版年
2024
NETL
NSTL
万方数据