Abstract
Small RNA(sRNA)triggered RNA silencing plays significant roles in viral resistance response by plants.Analysis of virus-derived sRNA profiles in plants is an efficient method for virus identification and de no-vo assembly of viral genomes.In this study,suspected virus-infected Hibiscus tiliaceus samples collected from Nanning were used for sRNA library construction and subsequent deep sequencing.After the assembly of total sR-NAs,H.tiliaceus leaves were found to be infected by hibiscus chlorotic mottle virus(HCMV).The library gene-rated about 11.77 million sRNA reads,of which 15 093 can be mapped onto viral genomes.Using de novo assem-bly and GenBank Virus RefSeq database blast,a candidate virus covers 18.8% of the full length genome nucleo-tide sequence of a badnavirus,which has the highest similarity to hibiscus bacilliform virus isolate GD-1(HBV-GD1).To confirm the existence of HCMV in the samples,a fragment of about 1 400 nucleotides encoding Re-verse transcriptase/RNase H was obtained by PCR,and confirmed by Sanger sequencing.This is the first report about using sRNA deep sequencing technology to identify the Hibiscus tiliaceus infecting virus,and this provides important information for further study on the molecular characteristics of HCMV and virus-host interactions.
基金项目
广西大学高层次人才助理教授科研启动经费项目(A3310051012)
NETL
NSTL
万方数据