Molecular identification of watermelon silver mottle virus infecting cucumber in Shandong
During a survey in 2022,cucumber displayed yellowing and chlorotic symptoms on leaves in Qing-zhou and Shouguang,Shandong Province.In order to investigate the virus species,eight samples were collec-ted,and total RNA was extracted from each sample.Three high quality RNA samples were mixed together for the next-generation sequencing(NGS).The NGS clean data were assembled into large contigs and then com-pared with the GenBank Virus RefSeq database using BLASTn software.The BLAST results were verified with reverse transcription-polymerase chain reaction(RT-PCR),confirming that the cucumber samples were infected by five species of virus,including watermelon silver mottle virus,cucumber mosaic virus,cucurbit aphid-borne yellows virus,zucchini yellow mosaic virus and watermelon mosaic virus.After cloning the full-length of S RNA(GenBank No.OQ184866),we found that the N gene of WSMoV was 828 nt in length,encoding 275 aa,sharing 92% -98% sequence identity with other WSMoV isolates.Further phylogenetic analysis showed that the N gene formed an independent cluster and was mostly related to the cucumber isolate.This is the first report that watermelon silver mottle virus infects cucumber in Shangdong Province.
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