Abstract
During a survey in 2022,cucumber displayed yellowing and chlorotic symptoms on leaves in Qing-zhou and Shouguang,Shandong Province.In order to investigate the virus species,eight samples were collec-ted,and total RNA was extracted from each sample.Three high quality RNA samples were mixed together for the next-generation sequencing(NGS).The NGS clean data were assembled into large contigs and then com-pared with the GenBank Virus RefSeq database using BLASTn software.The BLAST results were verified with reverse transcription-polymerase chain reaction(RT-PCR),confirming that the cucumber samples were infected by five species of virus,including watermelon silver mottle virus,cucumber mosaic virus,cucurbit aphid-borne yellows virus,zucchini yellow mosaic virus and watermelon mosaic virus.After cloning the full-length of S RNA(GenBank No.OQ184866),we found that the N gene of WSMoV was 828 nt in length,encoding 275 aa,sharing 92% -98% sequence identity with other WSMoV isolates.Further phylogenetic analysis showed that the N gene formed an independent cluster and was mostly related to the cucumber isolate.This is the first report that watermelon silver mottle virus infects cucumber in Shangdong Province.
基金项目
北京市农林科学院青年科研基金(QNJJ202131)
NETL
NSTL
万方数据