细辛篮状菌叶枯病病原鉴定及室内防控药剂筛选
Identification of the pathogen causing asarum leaf blight and screening of effective fungicides
刘坤 1孙文松 1张天静 1沈宝宇 1李晓丽1
作者信息
- 1. 辽宁省经济作物研究所,辽阳 111000;中国医学科学院药用植物研究所辽阳研究中心,辽阳 111000
- 折叠
摘要
采用组织分离法,对采自辽宁抚顺新宾的细辛叶枯病株进行病原分离和纯化.根据柯赫氏法则,对分离菌株进行致病性测定.通过形态学观察,结合rDNA-ITS序列分析,以及BenA与RPB2多基因位点序列对比分析,对病原菌进行鉴定.结果表明:经回接验证,明确分离所获菌株是细辛叶枯病的致病菌;代表性菌株XXY-2的形态特征以及在OA、MEA、DG18、YES、CYA和CREA等6种培养基上的菌落培养特征与Talaromyces brevis 一致;基于ITS-BenA-RPB2多基因序列系统发育分析结果显示,XXY-2与T.brevis的模式菌株DTO 307T和CBS 141833T处于同一分支,自展值为100,表明XXY-2为T.brevis.采用菌丝生长速率法,测定8种杀菌剂对T.brevis生长的抑制效果,其中吡唑醚菌酯和氟啶胺的室内抑菌效果最好,EC50值分别为0.0096和0.0056 μg,mL-1.这是T.brevis作为细辛叶枯病病原的首次报道,为后续开展细辛叶枯病综合防治奠定了理论基础.
Abstract
Asarum plants showing leaf blight symptoms were collected from Xinbin County,Fushun City,Liaoning Province,China.To investigate the causal agent of the disease,pathogenicity test was carried out with XXY-2,a representative fungal strain that were isolated from the diseased plant tissues,and the result showed that it is the pathogen causing asarum leaf blight.Based on morphological characters,strain XXY-2 was identi-fied as Talaromyces brevis.According to the results of multigene-combined(rDNA-ITS+BenA+RPB2)phyloge-netic analysis,strain XXY-2 was grouped into the same branch of T.brevis model strains DTO 307T and CBS 141833T,further confirming that it belongs to T.brevis.Indoor toxicity tests of 8 fungicides against strain XXY-2 showed that pyraclostrobin and fludioxonil had a better inhibitory effect on mycelial growth of the strain,with EC50 values of 0.0096 and 0.0056 μg·mL-1,respectively.This is the first report of T.brevis as a pathogen of asarum leaf blight,making a theoretical basis for integrated control of the disease.
关键词
细辛/叶枯病/致病性测定/多基因位点分析/药剂筛选Key words
Asarum/leaf blight/pathogenicity test/multilocus phylogeny/fungicide screening引用本文复制引用
基金项目
国家中药材产业技术体系建设项目辽阳综合试验站项目(CARS-21-06)
辽宁省农科院中药材学科建设项目(2019DD144221)
辽宁省科技厅民生计划(2021JH2/10200044)
辽宁省药用植物种质创新及综合利用重点实验室项目(2021JH13/10200007)
辽宁省应用基础研究计划(联合计划)项目(2022JH2/1013002840)
辽宁省农科院协同创新"揭榜挂帅"专项(2022XTCX0503)
辽宁省盖州市中药材科技特派团项目(2022JH5/10400066)
出版年
2024
NETL
NSTL
万方数据