首页|细辛篮状菌叶枯病病原鉴定及室内防控药剂筛选

细辛篮状菌叶枯病病原鉴定及室内防控药剂筛选

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采用组织分离法,对采自辽宁抚顺新宾的细辛叶枯病株进行病原分离和纯化.根据柯赫氏法则,对分离菌株进行致病性测定.通过形态学观察,结合rDNA-ITS序列分析,以及BenA与RPB2多基因位点序列对比分析,对病原菌进行鉴定.结果表明:经回接验证,明确分离所获菌株是细辛叶枯病的致病菌;代表性菌株XXY-2的形态特征以及在OA、MEA、DG18、YES、CYA和CREA等6种培养基上的菌落培养特征与Talaromyces brevis 一致;基于ITS-BenA-RPB2多基因序列系统发育分析结果显示,XXY-2与T.brevis的模式菌株DTO 307T和CBS 141833T处于同一分支,自展值为100,表明XXY-2为T.brevis.采用菌丝生长速率法,测定8种杀菌剂对T.brevis生长的抑制效果,其中吡唑醚菌酯和氟啶胺的室内抑菌效果最好,EC50值分别为0.0096和0.0056 μg,mL-1.这是T.brevis作为细辛叶枯病病原的首次报道,为后续开展细辛叶枯病综合防治奠定了理论基础.
Identification of the pathogen causing asarum leaf blight and screening of effective fungicides
Asarum plants showing leaf blight symptoms were collected from Xinbin County,Fushun City,Liaoning Province,China.To investigate the causal agent of the disease,pathogenicity test was carried out with XXY-2,a representative fungal strain that were isolated from the diseased plant tissues,and the result showed that it is the pathogen causing asarum leaf blight.Based on morphological characters,strain XXY-2 was identi-fied as Talaromyces brevis.According to the results of multigene-combined(rDNA-ITS+BenA+RPB2)phyloge-netic analysis,strain XXY-2 was grouped into the same branch of T.brevis model strains DTO 307T and CBS 141833T,further confirming that it belongs to T.brevis.Indoor toxicity tests of 8 fungicides against strain XXY-2 showed that pyraclostrobin and fludioxonil had a better inhibitory effect on mycelial growth of the strain,with EC50 values of 0.0096 and 0.0056 μg·mL-1,respectively.This is the first report of T.brevis as a pathogen of asarum leaf blight,making a theoretical basis for integrated control of the disease.

Asarumleaf blightpathogenicity testmultilocus phylogenyfungicide screening

刘坤、孙文松、张天静、沈宝宇、李晓丽

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辽宁省经济作物研究所,辽阳 111000

中国医学科学院药用植物研究所辽阳研究中心,辽阳 111000

细辛 叶枯病 致病性测定 多基因位点分析 药剂筛选

国家中药材产业技术体系建设项目辽阳综合试验站项目辽宁省农科院中药材学科建设项目辽宁省科技厅民生计划辽宁省药用植物种质创新及综合利用重点实验室项目辽宁省应用基础研究计划(联合计划)项目辽宁省农科院协同创新"揭榜挂帅"专项辽宁省盖州市中药材科技特派团项目

CARS-21-062019DD1442212021JH2/102000442021JH13/102000072022JH2/10130028402022XTCX05032022JH5/10400066

2024

10.13926/j.cnki.apps.001612

植物病理学报
中国植物病理学会

植物病理学报

CSTPCD北大核心
影响因子：0.865
ISSN：0412-0914
年,卷(期)：2024.54(2)
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