Isolation and identification of watermelon silver mottle orthotospovirus and cucum-ber mosaic virus infecting early spring watermelon in Yunnan Province
Watermelon planted in early spring is a characteristic fruit in hot area of Yunnan Province.Its viral diseases are more and more serious in recent years.To detect and identify the main viruses,the plants and fruits samples of watermelon were collected from Menghai county,Xishuangbanna state,and transmission electron microscope(TEM)observation,indirect enzyme-linked immunosorbent assay(ID-ELISA),RT-PCR amplifi-cation and analysis of viral genome sequences were carried out.The results showed that the viruses infecting watermelon were watermelon silver mottle virus(WSMoV)and cucumber mosaic virus(CMV).The detection rates of WSMoV and CMV using ID-ELISA were 70％and 20％,respectively,in leaf samples,and of complex infection was 15％.The detection rates of these two viruses by RT-PCR were 100％and 65％,respectively,and of complex infection was 65％.However,the complex infection rate of these two viruses detected by RT-PCR amplification could reach up to 100％in seeds.Phylogenetic analysis demonstrated that the genome sequences of WSMoV[21YV-40(GenBank accession no.:OP617563),21YV-43(GenBank accession no.:OP867047)]isolated in this study were similar to the squence of WSMoV[Banna-2011(GenBank accession no.:KM242056)]isolated from Yunnan watermelon in 2016,and the similarity was 99％.The genome sequences of CMV[CMVYN40(GenBank accession no.:OP617565),CMVYN46(GenBank accession no.:OP617566)]isolated in this study were similar to the sequence of CMV[A27(GenBank accession no.:FN552545)]isolated from watermelon in Thailand,and the similarity was 98％.This study demonstrated that the early spring watermelon was mainly infected by WSMoV and CMV,and complex infection was also common.The complex infection of WSMoV and CMV to both the plant and the seed of watermelon was reported for the first time.This study supplied a basis for virus prevention in the early spring watermelon.
early spring watermelonwatermelon silver mottle viruscucumber mosaic virusco-infectionseed
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