甘薯褪绿斑病毒山东分离物全基因组扩增及遗传进化分析
Complete genome sequence and phylogenetic analysis of sweet potato chlorotic fleck virus isolated from Shandong Province
韩志磊 1李光艳 2孙晓辉 1吴斌 1洪浩 1庞宗洋 3王树森 4辛志梅 1竺晓平 5姜珊珊6
作者信息
- 1. 山东省农业科学院植物保护研究所,山东省植物病毒学重点实验室,济南 250100
- 2. 山东省农业科学院植物保护研究所,山东省植物病毒学重点实验室,济南 250100;山东农业大学植物保护学院,山东省蔬菜病虫学重点实验室,泰安 271018
- 3. 山东省郯城县农业技术推广中心,郯城 276100
- 4. 山东省诸城市农业农村局,诸城 262200
- 5. 山东农业大学植物保护学院,山东省蔬菜病虫学重点实验室,泰安 271018
- 6. 山东省农业科学院植物保护研究所,山东省植物病毒学重点实验室,济南 250100;农业农村部植保生物技术重点实验室,宁波 315211
- 折叠
摘要
为了解甘薯褪绿斑病毒(sweet potato chlorotic fleck virus,SPCFV)在山东省的发生及遗传进化情况,分别于2019年和2020年采集了 131份甘薯样品,针对SPCFV进行病毒检测、全基因序列扩增、序列分析及遗传进化树构建.结果显示:SPCFV两年的检出率分别为7.1％和5.6％,所有SPCFV感染样品中皆为SPCFV与其他病毒的复合侵染.通过RACE和RT-PCR获得CFV-SD1和CFV-SD2两个全基因组序列,全长均为9 105 bp;与已报道分离物核苷酸序列一致性为72.9％～89.5％.全基因组遗传进化分析显示所有SPCFV分离物聚为两簇.且从不同样品中获得6个SPCFV山东分离物的外壳蛋白(coat protein,CP)序列,基于CP氨基酸序列开展遗传进化分析表明,山东分离物均归于亚洲类群的第一簇(Asian iso-lates1).氨基酸偏好性分析显示,CP氨基酸序列N端前35位同样存在多变区.本研究表明了山东省已成为SPCFV的常发区域,且病毒群体存在多样性,研究结果为指导SPCFV的有效防控提供了理论依据.
Abstract
To understand the occurrence and genetic evolution of sweet potato chorotic fleck virus(SPCFV)in Shandong province,China,131 sweet potato samples were collected for the detection of SPCFV in 2019 and 2020,and two complete sets of genome sequences were amplified and analyzed.The SPCFV detection rate in 2019 and 2020 was 7.1％and 5.6％,respectively.All positive samples were co-infected with SPCFV and other sweet potato viruses.The two complete sets of genome sequences(CFV-SD1,CFV-SD2)were obtained by RACE and RT-PCR,with a total length of 9 105 bp and a nucleotide similarity with the reported isolates was 72.9％to 89.5％.Phylogenetic analysis of genome sequences revealed that all SPCFV isolates clustered into two groups based on the CP genes of six Shandong isolates and that all Shandong isolates belong to the Asian group(Asian isolates 1).Amino acid preference analysis showed that the first 35 amino acids in the N-terminal region of the SPCFV CP protein exhibited variation.This study indicated that Shandong province has become a frequent occurrence area of SPCFV and the virus population was diverse.The results provide a theoretical basis for the effective prevention and control of SPCFV.
关键词
甘薯褪绿斑病毒/山东省/全基因组序列/外壳蛋白/遗传进化分析Key words
sweet potato chlorotic fleck virus/Shandong Province/complete genome sequence/coat protein/phylogenetic analysis引用本文复制引用
基金项目
山东省重点研发计划(2016GNC111003)
山东省农科院农业科技创新工程人才类任务项目(CXGC2022E04)
山东省自然科学基金(ZR2020QC128)
出版年
2024
NETL
NSTL
万方数据