Complete genome sequence and phylogenetic analysis of sweet potato chlorotic fleck virus isolated from Shandong Province
To understand the occurrence and genetic evolution of sweet potato chorotic fleck virus(SPCFV)in Shandong province,China,131 sweet potato samples were collected for the detection of SPCFV in 2019 and 2020,and two complete sets of genome sequences were amplified and analyzed.The SPCFV detection rate in 2019 and 2020 was 7.1％and 5.6％,respectively.All positive samples were co-infected with SPCFV and other sweet potato viruses.The two complete sets of genome sequences(CFV-SD1,CFV-SD2)were obtained by RACE and RT-PCR,with a total length of 9 105 bp and a nucleotide similarity with the reported isolates was 72.9％to 89.5％.Phylogenetic analysis of genome sequences revealed that all SPCFV isolates clustered into two groups based on the CP genes of six Shandong isolates and that all Shandong isolates belong to the Asian group(Asian isolates 1).Amino acid preference analysis showed that the first 35 amino acids in the N-terminal region of the SPCFV CP protein exhibited variation.This study indicated that Shandong province has become a frequent occurrence area of SPCFV and the virus population was diverse.The results provide a theoretical basis for the effective prevention and control of SPCFV.
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万方数据
