Biological function of the caspase FgCas4 in Fusarium graminearum
Caspase is known to be involved in modulating apoptosis in mammals,controlling the occurrence and development of various diseases.In present study,the null mutant ΔFgCas4 and complemented strain ΔFgCas4-C of the caspase gene FgCas4 were obtained by gene disruption and complementation respectively in Fusarium graminearum.We observed that the FgCas4 deletion mutant(ΔFgCas4)did not affect the growth rate,colony morphology,conidiation,virulence and DON production.However,the deletion mutant ΔFgCas4 exhibited more hyphal branching and percentage of conidia with 3 septa increased by 8.7％compared to wild type PH-1 and complemented strain ΔFgCas4-C.External environmental stress assays showed that the gene disrupt mutantΔFgCas4 became more sensitive to tested fungicides and metal ions.In addition,the lack of FgCas4 led to dra-matically increased lipid droplet biosynthesis as well as increased resistance to osmotic stress agents.Subcellular localization showed that the caspase FgCas4 localized in vacuoles.On the other,the loss of FgCas4 resulted in earlier process of autophagy.Taken together,our study provides evidences that the caspase FgCas4 of Fusarium graminearum plays important roles in asexual reproduction,various environmental stress responses and auto-phagy regulation.
NETL
NSTL
万方数据
