Transcriptome analysis of coronatine-induced resistance to Ralstonia solanacearum in tomato plants
The bacterial wilt caused by Ralstonia solanacearum has brought a serious threat to tomato production.Coronatine(COR),structurally similar to JA-Ile(JA-isoleucine),is a compound produced by Pseudomonas syringae.In this study,we observed that pre-treatment of tomato seedlings with COR alleviated the symptoms caused by R.solanacearum.To investigate how COR affects tomato resistance to R.solanacearum,transcriptome sequencing of tomato seedlings inoculated with the pathogen 24 h after COR treatment was carried out.Analysis of RNA-seq data showed that COR treatment induced a total of 2122 differentially expressed genes(DEGs),including 998 up-regulated genes and 1124 down-regulated genes.DEGs annotation and pathway en-richment were conducted using GO database and KEGG database,and the results showed that COR affected the expression of genes related to plant-pathogen interaction pathway and plant hormone signaling pathways.Mean-while,COR induced the up-regulation of genes involved in the jasmonic acid synthesis pathway and inhibited the expression of photosynthesis-related genes.Our results provide a theoretical basis for revealing the role of COR in plant-microbe interaction.