首页|WRR4C基因正调控拟南芥对橡胶树白粉菌和豇豆白粉菌的抗病性

WRR4C基因正调控拟南芥对橡胶树白粉菌和豇豆白粉菌的抗病性

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橡胶树白粉菌(Oidium heveae)与拟南芥野生型Col-0是一种非亲和相互作用,激发依赖于EDS1(ENHANCED DIS-EASE SUSCEPTIBILITY 1)和 PAD4(PHYTO ALEXIN DEFICIENT 4)的抗病性,推测 TIR-NB-LRR(TOLL INTERLEUKIN 1 RECEPTOR NUCLEOTIDE-BINDING LEUCINE-RICH REPEATS)类抗病基因参与对 O.heveae 的抗病性.本研究通过筛选针对O.heveae的TIR-NB-LRR类差异表达基因,发现WRR4C(white rust resistance 4 C)基因受O.heveae的诱导上调表达.经过表型分析鉴定,O.heveae可以在2个wrr4c单突变体上形成浓密的菌丝网络和少量的分生孢子.同时,O.heveae在 wrr4c突变体上激发的细胞死亡、胼胝体沉积和致病相关基因PR1(Pathogenesis related 1)的表达都显著降低,暗示着WRR4C基因正调控拟南芥对橡胶树白粉菌的抗病性.然而,通过白粉菌细胞进入率测定,WRR4C基因并不参与拟南芥对O.heveae的细胞进入抗性.进一步利用荧光定量PCR分析发现,WRR4C基因受O.heveae的诱导,在侵染后的48 h表达量达到最高,推测WRR4C基因主要在白粉菌细胞进入后发挥抗病功能.另外,通过接种豇豆白粉菌,发现WRR4C基因同时正调控拟南芥对豇豆白粉菌的抗病性.
WRR4C gene positively regulates the disease resistance of Arabidopsis to Oidium heveae and Erysiphe polygoni
Oidium heveae is an incompatible pathogen of Arabidopsis Col-0,and triggers disease resistance in Arabidopsis in an EDS1(ENHANCED DISEASE SUSCEPTIBILITY 1)and PAD4(PHYTOALEXIN DEFI-CIENT 4)dependent manner,suggesting that TIR-NB-LRR(TOLL INTERLEUKIN 1 RECEPTOR,NUCLEO-TIDE-BINDING,LEUCINE-RICH REPEAT)genes may involve in the disease resistance against O.heveae.In this study,the differentially expressed TIR-NB-LRR genes targeting O.heveae were screened,and the expres-sion of WRR4C(WHITE RUST RESISTANCE 4C)gene was found to up-regulated induced by O.heveae.Through inoculation assay,O.heveae was observed to develop dense hyphal network and a few conidia in two wrr4c single mutants,and triggered significantly decreased defense responses including cell death,callose depo-sition and PRI(Pathogenesis Related 1)gene expression,indicating that WRR4C positively regulated the disease resistance of Arabidopsis against the powdery mildew of Hevea brasiliensis.However,WRR4C gene did not par-ticipate in the cell pre-penetration resistance to O.heveae,and the expression of WRR4C reached to the highest level at 48 hours post inoculation,suggesting that WRR4C genes was mainly involved in the cell post-penetration resistance of Arabidopsis to O.heveae.In addition,we found WRR4C also positively regulated the disease resistance against Erysiphe polygoni in Arabidopsis.

ArabidopsisOidium heveaeResistance geneWRR4CErysiphe polygoni

王昊、戎伟

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海南大学植物保护学院,海口 570228

拟南芥 橡胶树白粉菌 抗病基因 WRR4C 豇豆白粉菌

国家自然科学基金

32060138

2024

植物病理学报
中国植物病理学会

植物病理学报

CSTPCD北大核心
影响因子:0.865
ISSN:0412-0914
年,卷(期):2024.54(2)
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