Identification of Fusarium oblongum causing trunk canker disease on Carya cathay-ensis
Trunk canker disease is one of the most common diseases of Carya cathayensis,causing huge eco-nomic losses.In May 2021,typical trunk canker symptoms were observed on hickory in Lin'an City,Zhejiang Province.Three fungal isolates(ZQ3,ZQ5 and ZQ12)were obtained by tissue isolation and purified using sin-gle-spore isolation method.Species identification was performed by morphological traits and sequences analyses of internal transcribed spacers(ITS)of rDNA,TEF1-α,and LSU gene,and all the fungal isolates were identi-fied as Fusarium oblongum.To fulfill the Koch's postulations,pathogenicity assay was carried out on phloem of the hickory stems.The all tested isolates were pathogenic to hickory,proving that F.oblongum was a pathogen inciting hickory trunk canker disease.This is the first report of F.oblongum causing trunk canker on Carya cathayensis in China.