Species identification of Fusarium causing root rot on soybean
Fungal disease is an important factor restricting the high and stable yield of soybean.In order to clarify the taxonomic status of pathogenic isolates SoyITS1 and SoyITS12 isolated from the diseased soybean roots with the symptoms of root rot,the two isolates were identified on the basis of morphological traits and sequence ana-lyses of translation elongation factor 1-α(EF1-α)and RNA polymerase Ⅱ beta subunit(RPB2).The results showed that the colonies of isolates SoyITS1 and SoyITS12 were milky white when they were cultured on PDA medium.After 7 d of culture on CLA medium,the conidia were falciform with blunt apical cells and slightly crooked base cells,with 3-4 septa,the size of which was(20-40)μmx(3.5-6.8)μm(n=50).BLAST sequence comparison showed that the consistency between strains SoyITS1 and SoyITS12 and Fusarium falci-forme was 86.7％-98.8％.In the two monogenic and polygenic phylogenetic trees,SoyITS1 and SoyITS12 strain was clustered in the same evolutionary branch as F.falciforme.Strain SoyITS1 and SoyITS12 were identi-fied as F.falciforme based on morphological characteristics,homology analysis and phylogenetic tree analysis.
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