Abstract
Wheat yellow dwarf disease causes typical disease symptoms such as yellowing,stunting,reduced seed size and yield,leading to severe economic loss in wheat production.Two barley yellow dwarf virus(BYDV)lines,BYDV-PAV and BYDV-GAV,are main viruses causing yellow dwarf in wheat.Maize yellow mosaic virus(MaYMV)was recently reported to cause yellow dwarf in wheat.In this study,diseased leaf sam-ples showing yellowing and stunting on wheat were collected in various regions in Henan Province in 2022.The optimal reaction condition for multiple RT-PCR detection of BYDV-PAV,BYDV-GAV and MaYMV was estab-lished with the following condition:final primer concentration at 0.1 μmol·L-1,annealing temperature at 55℃,annealing and extension time of 90 s and 120 s,respectively.We further used the established multiple RT-PCR system to detect 19 field samples.It showed that 11 0ut of 19 samples were positive for both BYDV-PAV and MaYMV,indicating that the mixed infection of BYDV-PAV and MaYMV was common in the field.Our study laid the foundation for the field surveillance and control of wheat yellow dwarf disease in Henan Province.
基金项目
河南省重点研发专项(231111111100)
河南省玉米产业技术体系建设项目植保岗位科学家科研专项(HARS-22-02-G3)
河南省青年人才托举工程项目(2022HYTP037)
河南农业大学科技创新项目(KJCX2021A13)
NETL
NSTL
万方数据