Establishment and application of multiple RT-PCR for detection of three yellow dwarf viruses
Wheat yellow dwarf disease causes typical disease symptoms such as yellowing,stunting,reduced seed size and yield,leading to severe economic loss in wheat production.Two barley yellow dwarf virus(BYDV)lines,BYDV-PAV and BYDV-GAV,are main viruses causing yellow dwarf in wheat.Maize yellow mosaic virus(MaYMV)was recently reported to cause yellow dwarf in wheat.In this study,diseased leaf sam-ples showing yellowing and stunting on wheat were collected in various regions in Henan Province in 2022.The optimal reaction condition for multiple RT-PCR detection of BYDV-PAV,BYDV-GAV and MaYMV was estab-lished with the following condition:final primer concentration at 0.1 μmol·L-1,annealing temperature at 55℃,annealing and extension time of 90 s and 120 s,respectively.We further used the established multiple RT-PCR system to detect 19 field samples.It showed that 11 0ut of 19 samples were positive for both BYDV-PAV and MaYMV,indicating that the mixed infection of BYDV-PAV and MaYMV was common in the field.Our study laid the foundation for the field surveillance and control of wheat yellow dwarf disease in Henan Province.
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