Identification of the pathogens of Rehmannia glutinosa viral disease and molecular variation analysis of the major viruses
A total of 60 leaf samples with mosaic and chlorotic symptoms were collected from the main planting areas of Rehmannia glutinosa in Henan province.High throughput sequencing and RT-PCR assays were used to detect viruses in the samples.The results showed that five virus species and one viroid were detected from the R.glutinosa samples of Henan province,including youcai mosaic virus(YoMV),rehmannia mosaic virus(ReMV),broad bean wilt virus 2(BBWV2),tobacco mild green mosaic virus(TMGMV),cucurbit chlorotic yellows virus(CCYV)and columnea latent viroid(CLVd).This is the first report of CLVd infection on R.glu-tinosa.All 60 samples were positive and the detection rates of YoMV,ReMV,BBWV2,TMGMV,CCYV and CLVd were 100％,93.3％,85.0％,78.3％,36.7％and 8.3％,respectively.All of these samples(100％)were found to be infected by two or more of the above viruses.The detection rate of co-infection by 3-6 kinds of viru-ses were 96.7％,and co-infection by 4 kinds of viruses were the major mixed type(40.0％).Combined infection by YoMV+ReMV+TMGMV+BBWV2 was the most common mixed type.Molecular variation analysis showed that CLVd was the most highly conserved,followed by YoMV and ReMV,but BBWV2 showed more variation.Phylogenetic tree analyses indicated that the isolates of YoMV and ReMV had a host specificity correlation,but no obvious host specificity and geographic differentiation correlation were found among the isolates of BBWV2 and CLVd.
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