假禾谷镰孢毒素化学型快速鉴定及在分析黄淮麦区分离物中的应用
A rapid method for chemotype identification of Fusarium pseudograminearum and its application on the analysis of the isolates from Huanghuai wheat-growing region of China
邓渊钰 1刘振国 2陈琛 3孙海燕 1曹淑琳 1李伟 1陈怀谷1
作者信息
- 1. 江苏省农业科学院植物保护研究所,南京 210014;江苏省粮食作物现代产业技术协同创新中心,扬州 225009
- 2. 江苏省农业科学院植物保护研究所,南京 210014
- 3. 江苏省农业科学院植物保护研究所,南京 210014;西湖大学生命科学学院,杭州 310024
- 折叠
摘要
假禾谷镰孢(Fusarium pseudograminearum)是我国小麦茎基腐病的优势病原菌之一,为了快速简便地鉴定其毒素化学型,本研究检验了 4种已报道的镰孢菌毒素化学型分子生物学鉴定方法在该菌上的适用性,结果显示这些方法均不能有效区分它的三种化学型.基于此,本研究设计特异性引物,并建立了能用于F.pseudograminearum毒素化学型分子生物学鉴定的方法.采用此方法,对从河北、山东、河南和江苏采集的479株F.pseudograminearum的化学型进行了鉴定,发现这些菌株中只有极少数为NIV型,其余均为DON型.河北、山东和河南的F.pseudograminearum以15-ADON化学型为主,而江苏的菌株中3-ADON化学型则占多数.F.pseudograminearum的3-ADON和15-ADON化学型的地理分布与禾谷镰孢复合种相似.
Abstract
Fusarium pseudograminearum is one of the dominant pathogens causing wheat crown rot in China.To identify its chemotypes quickly and simply,the 4 reported methods for Fusarium chemotype identification were tested for their applicability to this pathogen.The results showed that these methods could not effectively differentiate the three chemotypes of F.pseudograminearum.In this case,we explored a new method to identify the chemotypes of F.pseudograminearum by designing specific primers.Using this new developed method,the chemotypes of 479 strains of F.pseudograminearum collected from Hebei,Shandong,Henan and Jiangsu prov-inces,China were identified.Besides rare NIV producers,theses isolates are mainly DON producers.Most iso-lates from Hebei,Shandong and Henan provinces belong to 15-ADON in chemotype,but the isolates from Jian-gsu Province primarily belong to the chemotype of 3-ADON.The geographic distribution of the 3-ADON and 15-ADON isolates of F.pseudograminearum is similar to that of F.graminearum species complex.
关键词
小麦茎基腐病/假禾谷镰孢/毒素化学型/特异性引物/3-ADON/15-ADON/NIVKey words
crown rot of wheat/Fusarium pseudograminearum/chemotype/specific primer/3-ADON/15-ADON/NIV引用本文复制引用
基金项目
国家现代农业产业技术体系项目(CARS-03)
出版年
2024
NETL
NSTL
万方数据