A rapid method for chemotype identification of Fusarium pseudograminearum and its application on the analysis of the isolates from Huanghuai wheat-growing region of China
Fusarium pseudograminearum is one of the dominant pathogens causing wheat crown rot in China.To identify its chemotypes quickly and simply,the 4 reported methods for Fusarium chemotype identification were tested for their applicability to this pathogen.The results showed that these methods could not effectively differentiate the three chemotypes of F.pseudograminearum.In this case,we explored a new method to identify the chemotypes of F.pseudograminearum by designing specific primers.Using this new developed method,the chemotypes of 479 strains of F.pseudograminearum collected from Hebei,Shandong,Henan and Jiangsu prov-inces,China were identified.Besides rare NIV producers,theses isolates are mainly DON producers.Most iso-lates from Hebei,Shandong and Henan provinces belong to 15-ADON in chemotype,but the isolates from Jian-gsu Province primarily belong to the chemotype of 3-ADON.The geographic distribution of the 3-ADON and 15-ADON isolates of F.pseudograminearum is similar to that of F.graminearum species complex.
crown rot of wheatFusarium pseudograminearumchemotypespecific primer3-ADON15-ADONNIV
NETL
NSTL
万方数据
