Detection of viruses infecting watermelon and genomic analysis of watermelon mosaic virus in Jiyuan of Henan Province
In 2022,a study investigating viral diseases in three watermelon planting plots was conducted in Jiyuan,revealing an average incidence rate of approximately 97％.Twenty samples,comprising 14 watermelon samples,4 melon samples,1 pumpkin sample and 1 pigweed(Amaranthaceae retroflexus L.),were collected from the field.Three watermelon samples and one Amaranthaceae weed sample underwent small RNA sequencing,separately,and virus detect identified seven viruses from the four small RNA sequencing data sets.Subsequently,RT-PCR using primer pairs specific for 16 watermelon-infecting viruses identified the same seven viruses in all 20 samples.Watermelon mosaic virus(WMV)and zucchini yellow mosaic virus(ZYMV)had de-tection rates of 100％and 85％,respectively,making them the two most prevalent viruses in watermelon fields.WMV was also identified in pigweed,which could serve as an intermediate host of WMV in the field.The full genome sequences of four WMV isolates were derived from four data sets of small RNA sequencing.Comparing the identity and conducting a phylogenetic analysis based on the full genome sequences of WMV revealed that WMV isolates from Jiyuan have very high genetic diversity,suggesting that there could be multiple initial infection sources of WMV in the region.This study identified the causal agents of viral diseases in watermelon in Jiyuan;established a theoretical foundation for developing prevention and control strategies and conducting epi-demiological research on viral diseases in watermelon in the region.
NETL
NSTL
万方数据
