Population genetic diversity analysis of tomato zonate spot virus on tobacco in Yunnan based on NP gene sequence
To investigate the incidence and population genetic diversity of tomato zonate spot virus(TZSV)on tobacco in Yunnan,560 samples of tobacco plants exhibiting symptoms of spot wilt were collected from various tobacco regions in this province.A pair of specific primers from the nucleocapsid protein(NP)gene were used to screen the samples.NP gene of the selected TZSV-positive samples was cloned,sequenced,and employed for phylogenetic,analyses,assessment of genetic diversity and determination of population differentiation.The re-sults of RT-PCR showed that 262 out of the 560 samples were found to be infected with TZSV,with a mean pos-itive detection rate of 46.8％.A significant recombination signal within the genome of the DX_LJHP_65 isolate was identified through recombination analysis.Phylogenetic analysis showed that 129 TZSV isolates can be divid-ed into 6 groups,which tended to cluster according to their geographical origin,suggesting that the evolution of TZSV exhibits a pronounced geographical specificity.The mismatch distribution analysis results for the TZSV population in Yunnan.indicated a multimodal distribution,suggesting that it has not undergone recent demo-graphic expansion.The analysis of genetic differentiation among populations revealed a significant difference in gene exchange patterns.Specifically,the frequency of gene flow between the DX and DDB populations and oth-er populations is found to be very low.On the other hand,there was frequent gene exchange observed between the remaining populations.This study is the first report on the genetic variation of TZSV isolate populations in various tobacco regions of Yunnan.It offers crucial insights that serve as a valuable reference for the prevention and control of TZSV.
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