摘要
由于绝大多数水稻亲本的稻瘟病抗性基因组成不清楚,等位基因间的高度同源,导致分子标记辅助选择技术在水稻抗病育种中的应用受到限制.本研究首先分析了稻瘟病抗性基因Pik位点11个等位基因编码区的核苷酸序列,筛选出Pikp-1、Pikh-1、Pik-1、Pikm-1和Pi1-1特异的多态性位点,再与水稻资源中心(Rice Resource Center)中155个水稻基因组进行比对分析,在序列特异性最强的位置设计引物,成功地为这5个等位基因开发出了特性的分子标记.以24个抗稻瘟病单基因系为对照,应用这些分子标记,明确了 109个水稻亲本中含有Pik-1、Pikh-1、Pikp-1、Pi1-1和Pikm-1基因的材料分别有0、1、5、14和20个.对'绵恢365'中的Pi1基因测序,验证了分子标记的有效性及准确性.本研究为Pik位点在水稻抗稻瘟病育种中的应用提供了明确的亲本资源和可靠的分子标记.
Abstract
The application of molecular marker assisted breeding is limited in rice,which due to unclear resist-ance gene composition in most rice parent materials and the highly homology of allelic genes.In this study,we designed the functional nucleotide polymorphism markers for Pikp-1,Pikh-1,Pik-1,Pikm-1 and Pi1-1 at the specific polymorphism sites,respectively.Those sites were screened by the allelic genes alignment and then picked out the most specific sites by blasting with 155 rice genomes at Rice Resource Center.Based on those markers,we examined 24 monogenic lines and 109 rice parent lines which using for rice breeding in Sichun ba-sin,and finally identified Pik-1,Pikh-1,Pikp-1,Pi1-1,and Pikm-1 gene from 0,1,5,14,and 20 rice parent lines.Among them,the Pi1 gene was identified by sequencing in'Mianhui 365',which verified the functional markers can distinguish those allelic genes at Pik site,effectively and accurately.It identified a number of rice parent resources with definite resistance gene composition at Pik site and provided reliable molecular markers for Pik-1,Pikh-1,Pikp-1,Pi1-1,and Pikm-1 gene.
基金项目
国家自然科学基金(U19A2033)
国家自然科学基金(31901839)
四川省青年科技创新研究团队(2022JDTD0023)
NETL
NSTL
万方数据