Development and application of specific molecular markers for five allelic rice blast resistance genes in Pik gene site in rice
The application of molecular marker assisted breeding is limited in rice,which due to unclear resist-ance gene composition in most rice parent materials and the highly homology of allelic genes.In this study,we designed the functional nucleotide polymorphism markers for Pikp-1,Pikh-1,Pik-1,Pikm-1 and Pi1-1 at the specific polymorphism sites,respectively.Those sites were screened by the allelic genes alignment and then picked out the most specific sites by blasting with 155 rice genomes at Rice Resource Center.Based on those markers,we examined 24 monogenic lines and 109 rice parent lines which using for rice breeding in Sichun ba-sin,and finally identified Pik-1,Pikh-1,Pikp-1,Pi1-1,and Pikm-1 gene from 0,1,5,14,and 20 rice parent lines.Among them,the Pi1 gene was identified by sequencing in'Mianhui 365',which verified the functional markers can distinguish those allelic genes at Pik site,effectively and accurately.It identified a number of rice parent resources with definite resistance gene composition at Pik site and provided reliable molecular markers for Pik-1,Pikh-1,Pikp-1,Pi1-1,and Pikm-1 gene.
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