Isolation,identification and resistance evaluation of strawberry angular leaf spot pathogen in Yunnan Province
Strawberry angular leaf spot is one of the important bacterial diseases in Yunnan in recent years.In this study,a bacterial strain isolated from the strawberry leaf lesion was identified as Xanthomonas fragariae based on morphological,physiological and biochemical analysis,pathogenicity detection,as well as the phylo-genetic analysis of the 16S rDNA and hrpB genes.The physiological and biochemical detection showed that the pathogenic bacterium could utilize 26 carbon sources such as D-maltose,D-trehalose,D-cellobiose,sucrose and D-pintobiose.It can react with seven chemical sensitive substances such as nalidixic acid and lincomycin.The pathogenic bacterium had weak salt tolerance,sensitive to acid,and could use organic acids such as L-lactic acid and citric acid.The disease resistance evaluation experiment showed that'Benihoppeand'and'Sweet Charlie'were highly resistant varieties,with disease indexes of 5.95 and 4.76,respectively,and the proportion of highly resistant varieties was 14.28％.The moderately resistant varieties included'Portola','All star','Yuexiu','Snow White','Jingzangxiang',and'Xiangye',and the proportion of moderately resistant varieties was 42.85％.In conclusion,we identified the pathogenic bacterium of strawberry angular leaf spot,and obtained several disease-resistant strawberry varieties through screening.These results laid a foundation for further research on the characteristics of disease occurrence and prevention methods.
strawberryisolation and identification of pathogensXanthomonas fragariaeresistance evalua-tion
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