Screening of the differential host spectrum of southern corn rust and its application in the study of pathogenic type
Southern corn rust caused by Puccinia polysora Underw.,is a fulminant and airborne disease,which can cause serious losses during epidemic years.Many studies at home and abroad have shown that the physiologi-cal races of P.polysora were very abundant,and the epidemic of plant diseases was mainly caused by the domi-nant races of P.polysora,but there were few research reports on the physiological races of P.polysora in China,so it brings many difficulties to the prediction and prevention of this disease,and has become a bottleneck in corn production.Based on the current research on the resistance genes and resistant inbred lines of southern corn rust in China,this study selected inbred lines containing known resistance genes and backbone inbred lines in maize production as differrential to be identified.Isolates of southern corn rust collected from different regions were used to identify these inbred lines resistance at the seeding in greenhouses,and the virulence frequency(VF)of different inbred lines.Then the inbred lines with the ability to identify different pathotype were selected to construct a set of differrential,and the pathotypes of southern corn rust in China was studied.14 pathotypes were identified.Among them,pathotype 1,which can only infect Huangzaosi,with the highest frequency of 0.58,was the dominant pathotype of P.polysora in China,which could be found from 7 provinces such as Hainan,Guangxi,Guangdong,shandong,etc.in 2016 and 2017.This pathotype,which can be determined as a new physiological race of southern corn rust pathogen in China.This is the first physiological race of P.polysora identified in China,temporarily named as Nanzhong1(NZ1).