Specific PCR detection of Colletotrichum asianum causing mango anthracnose
Colletotrichum asianum is one of the most important pathogens that cause anthracnose on mango plants.To establish a method for rapid detection of C.asianum,a C.asianum-specific sequence was obtained by comparing genomic sequences of different Colletotrichum species,and a pair of specific primers:caf1(5'-CCGTCAGACGGAATTATCAGC-3')and car1(5'-CCGATCCTGTCTTTGAAATGG-3')was designed accor-dingly.Specificity test results showed that a 419-bp target amplicon could only be obtained from C.asianum rather than other Colletotrichum species and non-Colletotrichum fungal and bacterial species that we tested in this study using the primer pair caf1/car1,with a detection sensitivity of 1 pg-μL-1.The 419-bp amplicon could be detected from both mango leaves artificially inoculated with C.asianum strain YN55-1 and from naturally infec-ted mango leaves with anthracnose symptoms.The specific PCR system developed in this study provides a tech-nical support for monitoring and rapid diagnosis of mango anthracnose caused by C.asianum in the field.
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