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砀山酥梨炭疽病病原鉴定和检测及致病性分析

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Identification,detection and pathogenicity analysis of'Dangshan Su'pear anthrac-nose
In recent years,pear anthracnose has become one of the major pear diseases in Dangshan County of Anhui Province and its surrounding areas.In order to identify the pathogens of'Dangshan Su'pear anthracnose,the diseased samples were collected from Dangshan County and the pathogens were isolated,purified,and iden-tified through morphology and molecular biology analysis.In this study,the pathogenicity of the pathogens was assayed by using the fruits and leaves of'Dangshan Su'pear as materials.All these confirmed that the patho-gens were mainly Colletotrichum fructicola and C.siamense.The specific detection primers Cf-F/Cf-R were de-signed according to part of the mRNA sequence of C.fructicola,which could specifically detect C.fructicola from similar strains and diseased tissues.

'Dangshan Su'pearanthracnosepathogen identificationPCR detectionpathogenicity

周贝贝、高正辉、杨雪、石旺鹏、齐永杰、马娜、阚丽平、徐义流

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中国农业大学植物保护学院,北京 100193

安徽省农业科学院园艺研究所,合肥 230001

安徽省农业科学院植物保护与农产品质量安全研究所,合肥 230001

园艺作物种质创制及生理生态安徽省重点实验室,合肥 230001

农业农村部园艺作物种质创制与利用重点实验室(部省共建),合肥 230001

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'Dangshan Su'pear anthracnose pathogen identification PCR detection pathogenicity

农业农村部园艺作物种质创制与利用重点实验室(部省共建)园艺作物种质创制及生理生态安徽省重点实验室国家梨产业技术体系专项资金资助国家重点研发计划研究任务安徽省农业科学院人才项目安徽省农业科学院人才项目安徽省农业科学院平台项目

CARS-282019YFD1001404-1-3LJRC-202108QNYC-2021122023YL015

2024

10.13926/j.cnki.apps.001341

植物病理学报
中国植物病理学会

植物病理学报

CSTPCD北大核心
影响因子：0.865
ISSN：0412-0914
年,卷(期)：2024.54(3)