砀山酥梨炭疽病病原鉴定和检测及致病性分析
Identification,detection and pathogenicity analysis of'Dangshan Su'pear anthrac-nose
周贝贝 1高正辉 2杨雪 3石旺鹏 4齐永杰 2马娜 2阚丽平 5徐义流2
作者信息
- 1. 中国农业大学植物保护学院,北京 100193;安徽省农业科学院园艺研究所,合肥 230001;安徽省农业科学院植物保护与农产品质量安全研究所,合肥 230001
- 2. 安徽省农业科学院园艺研究所,合肥 230001;园艺作物种质创制及生理生态安徽省重点实验室,合肥 230001;农业农村部园艺作物种质创制与利用重点实验室(部省共建),合肥 230001
- 3. 安徽省农业科学院植物保护与农产品质量安全研究所,合肥 230001
- 4. 中国农业大学植物保护学院,北京 100193
- 5. 安徽省农业科学院园艺研究所,合肥 230001
- 折叠
Abstract
In recent years,pear anthracnose has become one of the major pear diseases in Dangshan County of Anhui Province and its surrounding areas.In order to identify the pathogens of'Dangshan Su'pear anthracnose,the diseased samples were collected from Dangshan County and the pathogens were isolated,purified,and iden-tified through morphology and molecular biology analysis.In this study,the pathogenicity of the pathogens was assayed by using the fruits and leaves of'Dangshan Su'pear as materials.All these confirmed that the patho-gens were mainly Colletotrichum fructicola and C.siamense.The specific detection primers Cf-F/Cf-R were de-signed according to part of the mRNA sequence of C.fructicola,which could specifically detect C.fructicola from similar strains and diseased tissues.
关键词
'Dangshan Su'pear/anthracnose/pathogen identification/PCR detection/pathogenicityKey words
'Dangshan Su'pear/anthracnose/pathogen identification/PCR detection/pathogenicity引用本文复制引用
基金项目
农业农村部园艺作物种质创制与利用重点实验室(部省共建)()
园艺作物种质创制及生理生态安徽省重点实验室()
国家梨产业技术体系专项资金资助(CARS-28)
国家重点研发计划研究任务(2019YFD1001404-1-3)
安徽省农业科学院人才项目(LJRC-202108)
安徽省农业科学院人才项目(QNYC-202112)
安徽省农业科学院平台项目(2023YL015)
出版年
2024
NETL
NSTL
万方数据