Differential analysis on mating type distribution and fertility capacity of Magna-porthe oryzae of Liaoning Province
Rice blast fungus has diverse polymorphism in paddy fields.To clarify the role of genetic recombina-tion on biodiversity of Magnaporthe oryzae population in paddy fields,336 single-spore isolates of M.oryzae were collected from five main rice-producing regions of Liaoning Province,and their difference on mating type distribution and fertility capacity were analyzed.The PCR amplification on mating type genes showed that all 336 isolates belonged to the same mating type,MAT1-2.The confrontation cultivations of all isolates with a standard strain P9 having an opposite mating type(MAT1-1)showed that the average proportion of fertile strains was 37.5％and the average number of perithecia of each cross was 38.8.Moreover,the fertility capacities of the iso-lates from the five main rice-producing regions were significantly varied.Taken together,these findings suggest that sexual reproduction of M.oryzae population in Liaoning Province is rare or probably non-existent though they retain certain fertility capacities,and the biodiversity of M.oryzae population in paddy fields may be attrib-uted by other factors.
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