灰葡萄孢分泌蛋白BcSGP1的功能研究
Functional study of Botrytis cinerea secreted protein BcSGP1
宋宣同 1聂晓飞 1王紫瑶 1毕凯 1朱闻君1
作者信息
- 1. 武汉轻工大学生命科学与技术学院,武汉 430023
- 折叠
摘要
由灰葡萄孢(Botrytis cinerea)引起的灰霉病害可造成农作物严重减产,而分泌蛋白在灰葡萄孢的侵染过程中起着重要作用.但迄今为止,对灰葡萄孢分泌蛋白功能和作用机理的研究仍有待进一步的深入.本研究从灰葡萄孢侵染时期的分泌蛋白组中筛选到1个分泌蛋白BcSGP1,该基因在灰葡萄孢侵染期上调表达.将BcSGP1基因敲除后,基因敲除转化子的致病力降低,但生长速度、产孢量和抗逆性没有变化.通过农杆菌注射法在本氏烟草叶片中瞬时表达BcSGP1后能引起叶片坏死,并且其诱导植物坏死的活性依赖于植物中的类受体激酶BAK1,但不依赖于类受体激酶SOBIR1.此外,BcSGP1还能诱导植物对灰葡萄孢的抗病性.以上结果表明,BcSGP1是一个致病相关分泌蛋白,并参与诱导植物的抗性,在灰葡萄孢与植物互作中起着重要作用.研究结果增强了对灰葡萄孢致病机制的理解,为灰霉病害的有效防治提供理论依据和基因资源.
Abstract
Gray mold disease caused by Botrytis cinerea leads to severe crop yield reduction,and the secreted proteins play significant roles in the fungal infection.However,the functional mechanisms of these secreted proteins in B.cinerea remain largely unknown.In this study,a secreted protein,BcSGP1,from the secretome of B.cinerea during infection stages was identified.The expression level of BcSGP1 was upregulated during infec-tion stages.Deletion of BcSGP1 caused reduction in pathogenicity,but not in growth rate,conidial production,or stress resistance.Transient expression of BcSGP1 in Nicotiana benthamiana leaves using agroinfiltration induced necrosis,and this necrosis-inducing activity depended on the plant receptor-like kinase BAK1,but not the SOBIR1.Furthermore,BcSGP1 could induce resistance against B.cinerea in N.benthamiana leaves.These results suggest that BcSGP1 is a pathogenesis-related secreted protein and involved in inducing plant resistance during the interaction between B.cinerea and plants.This study enhances our understanding of the pathogenic mechanisms of B.cinerea,providing a theoretical basis and genetic resources for effective control of gray mold disease.
关键词
灰葡萄孢/分泌蛋白/BcSGP1/致病力Key words
Botrytis cinerea/secreted proteins/BcSGP1/pathogenicity引用本文复制引用
出版年
2024
NETL
NSTL
万方数据