Functional study of Botrytis cinerea secreted protein BcSGP1
Gray mold disease caused by Botrytis cinerea leads to severe crop yield reduction,and the secreted proteins play significant roles in the fungal infection.However,the functional mechanisms of these secreted proteins in B.cinerea remain largely unknown.In this study,a secreted protein,BcSGP1,from the secretome of B.cinerea during infection stages was identified.The expression level of BcSGP1 was upregulated during infec-tion stages.Deletion of BcSGP1 caused reduction in pathogenicity,but not in growth rate,conidial production,or stress resistance.Transient expression of BcSGP1 in Nicotiana benthamiana leaves using agroinfiltration induced necrosis,and this necrosis-inducing activity depended on the plant receptor-like kinase BAK1,but not the SOBIR1.Furthermore,BcSGP1 could induce resistance against B.cinerea in N.benthamiana leaves.These results suggest that BcSGP1 is a pathogenesis-related secreted protein and involved in inducing plant resistance during the interaction between B.cinerea and plants.This study enhances our understanding of the pathogenic mechanisms of B.cinerea,providing a theoretical basis and genetic resources for effective control of gray mold disease.
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