Exploring key pathogenic genes in Fusarium graminearum based on genetic diver-sity and selection elimination analysis
In recent years,wheat scab,corn stalk rot and ear rot caused by Fusarium graminearum have led to substantial losses in crop yields.To investigate the genetic diversity and identify pathogenicity-related genes of F.graminearum,we performed population genetic diversity analysis and selective elimination analysis on 93 F.gra-minearum strains with released genome-wide resequencing data,using single nucleotide polymorphism(SNP)technology.The resequencing data of these F.graminearum strains were meticulously processed by using the Ge-nome Analysis Toolkit 4(GATK4),yielding a collection of 3,817,652 SNP markers.Based on these markers,a phylogenetic tree was constructed,and principal component analysis(PCA)and population structure analysis were conducted,effectively partitioning the 93 F.graminearum into 3 distinct groups.The selection elimination analysis of group 1 and group 2 revealed that group 1 exhibited a more pronounced response to selection pressure.A total of 70 regions were identified as candidate sites within the top 5％intersection region of population poly-morphism(θπ)and population differentiation index(Fst).Furthermore,76 protein-coding genes were identi-fied in F.graminearum by leveraging the genomic location information.The results of Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis showed that these 76 candidate genes are mainly in-volved in metabolic pathways.Among them,eight genes(FGSG_05447,FGSG_05610,FGSG_10272,FGSG_10313,FGSG_01353,FGSG_05545,FGSG_10858 and FGSG_12745)are closely related to the pathogenicity of F.graminearum through further gene expression analysis.The result lays a basis for clarifying the pathogenic mechanism of F.graminearum and breeding F.graminearum-resistant wheat and maize varieties.
NETL
NSTL
万方数据
