Jasmonic acid biosynthetic genes positively regulate age-related resistance of tomato plants to Phytophthora infestans
Late blight caused by Phytophthora infestans seriously affects the yield and quality of tomato.Previ-ous research found that tomato plants contain age-related resistance(ARR)to P.infestans,but the underlying mechanisms are still unclear.Here,we used tomato variety'Micro Tom'as the tested material and found that younger(4-week-old)plants are more resistant while older(8-week-old)plants are more susceptible to late blight.Through RNA sequencing and real-time quantitative PCR(qPCR)analysis,we observed that the tran-scription levels of genes involved in jasmonic acid(JA)synthesis,such as AOS1,AOS2 and AOC,are higher in 4-week-old plants than those in 8-week-old plants.We further examined the levels of several phytohormones and found that the concentration of JA in 4-week-old plants is significantly higher than that in 8-week-old plants.Transient expression of AOS1,AOS2 or AOC in tobacco leaves made them more resistant to late blight,sugges-ting that these JA biosynthetic genes can enhance tomato resistance to late blight.Tomato plants sprayed with MeJA were more resistant whereas tomato plants sprayed with JA synthesis inhibitor DIECA were more suscepti-ble to late blight,suggesting that JA positively regulates tomato resistance to late blight.Thus,we provide evidence supporting a model in which genes involved in JA synthesis play important roles in the age-related re-sistance to late blight in tomato.Our results lay an important basis for using ARR to control tomato late blight.
tomato late blightage-related resistance(ARR)jasmonic acid(JA)AOSAOC
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