水稻恢复系'雅恢2115'含有抗稻瘟病基因Pib
The elite rice restorer line'Yahui2115'carries blast resistance gene Pib
胡小红 1李恒进 2石军 3李德强 1樊晶 1黄富 4王文明1
作者信息
- 1. 四川农业大学西南作物基因资源发掘与利用国家重点实验室,成都 611130
- 2. 宜宾市农业科学院,宜宾 644600
- 3. 绵阳市农业科学研究院/厅市共建作物特色资源创制及应用四川省重点实验室,绵阳 621023
- 4. 四川农业大学农学院,成都 611130
- 折叠
摘要
稻瘟病对水稻生产具有毁灭性的危害.选育和利用抗病品种是控制稻瘟病最经济有效且环保的方法.明确抗病品系的抗病基因将有利于选育新的抗病品种及品种合理布局.'雅恢2115'是广谱高抗稻瘟病的优良恢复系,前期研究表明其中含有抗瘟基因Pi2.为了鉴定'雅恢2115'中其他抗瘟基因,本研究利用对Pi2单基因系致病的稻瘟菌菌株M14Ga089和'丽江新团黑谷/雅恢2115'的F2代群体进行抗瘟基因定位分析.结果发现F2代群体的抗/感比率符合3∶1的显性单基因分离比,表明'雅恢2115'对菌株M14Ga089的抗性由1个显性基因控制,将其命名为Pir2115-1(t).通过极端隐性群体法将Pir2115-1(t)基因定位到第2号染色体RM6307和RM208之间,该区域包含一个已克隆的抗瘟基因Pib.基因表达分析发现在'雅恢2115'中Pib受稻瘟菌侵染高度诱导表达,序列分析发现'雅恢2115'中Pib编码的蛋白质序列与已报道的Pib完全一致,表明'雅恢2115'中含有Pib.本研究结果将为'雅恢2115'在育种中的运用及其组合的品种布局提供重要依据.
Abstract
Rice blast disease is destructive to rice production.Breeding and utilization of resistant varieties are the most economical,effective and environmentally friendly methods to control the rice blast.Identifying the re-sistance genes of resistant varieties will be beneficial to the selection of new resistant varieties and the distribution of resistant varieties.'Yahui2115'is an elite rice restorer line with broad-spectrum resistance to rice blast.Previ-ous studies have shown that'Yahui2115'contains Pi2.In order to identify other rice blast resistance genes in'Yahui2115',we used the strain M14Ga089 virulent to the monogenic line harboring Pi2 to analyze the segrega-tion of blast resistance in the F2 population of'LTH/Yahui2115'.The results showed that the resistance/suscep-tibility ratio was 3∶1 in the F2 population,indicating that the resistance from'Yahui2115'to strain M14Ga089 was controlled by one dominant gene,which was named Pir2115-1(t).Through extreme recessive population method,Pir2115-1(t)was mapped between SSR markers RM6307 and RM208 on chromosome 2,a locus that contains a known blast resistance gene,Pib.Gene expression analysis showed that Pib in'Yahui2115'was highly induced by Magnaporthe oryzae infection,and sequence analysis showed that the protein sequence encoded by Pib in'Yahui2115'was completely identical to the reported Pib,indicating that'Yahui2115'car-ries Pib.This study provides the basis for the application of'Yahui2115'in breeding and the variety distribution of its combinations.
关键词
水稻/恢复系/稻瘟病/抗病基因Key words
rice/restorer line/rice blast/resistance gene引用本文复制引用
基金项目
国家自然科学基金(U19A2033)
国家自然科学基金(32301838)
四川省青年科技创新研究团队项目(2022JDTD0023)
四川省自然科学基金(2022NSFSC1715)
出版年
2024
NETL
NSTL
万方数据