The elite rice restorer line'Yahui2115'carries blast resistance gene Pib
Rice blast disease is destructive to rice production.Breeding and utilization of resistant varieties are the most economical,effective and environmentally friendly methods to control the rice blast.Identifying the re-sistance genes of resistant varieties will be beneficial to the selection of new resistant varieties and the distribution of resistant varieties.'Yahui2115'is an elite rice restorer line with broad-spectrum resistance to rice blast.Previ-ous studies have shown that'Yahui2115'contains Pi2.In order to identify other rice blast resistance genes in'Yahui2115',we used the strain M14Ga089 virulent to the monogenic line harboring Pi2 to analyze the segrega-tion of blast resistance in the F2 population of'LTH/Yahui2115'.The results showed that the resistance/suscep-tibility ratio was 3∶1 in the F2 population,indicating that the resistance from'Yahui2115'to strain M14Ga089 was controlled by one dominant gene,which was named Pir2115-1(t).Through extreme recessive population method,Pir2115-1(t)was mapped between SSR markers RM6307 and RM208 on chromosome 2,a locus that contains a known blast resistance gene,Pib.Gene expression analysis showed that Pib in'Yahui2115'was highly induced by Magnaporthe oryzae infection,and sequence analysis showed that the protein sequence encoded by Pib in'Yahui2115'was completely identical to the reported Pib,indicating that'Yahui2115'car-ries Pib.This study provides the basis for the application of'Yahui2115'in breeding and the variety distribution of its combinations.
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