首页|黄精轮作改善土壤微生物群落缓解三七连作障碍潜力分析

黄精轮作改善土壤微生物群落缓解三七连作障碍潜力分析

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为探究黄精轮作后对三七连作土壤微生物群落的影响,评估轮作及自然休耕两种方式的修复能力.本研究以三七二年连作土壤为对象,在三七连作后种植二年黄精,并以同等条件土壤休耕后再复种三七,基于微生物组学结合生信分析技术研究黄精轮作对三七连作土壤微生物结构组成及菌群变化的影响.结果显示:黄精轮作后复种三七一年存苗率可达88.33%,显著高于休耕处理;三七根腐发病率均低于20%,但相较于休耕处理差异不显著.黄精轮作后土壤真菌及细菌多样性均显著降低(P<0.01),主坐标分析结果表明:轮作能显著影响三七连作土壤真菌及细菌β多样性(P<0.001);轮作能显著增加子囊菌门相对丰度,降低担子菌门、被孢霉门真菌相对丰度,轮作后细菌厚壁菌门、芽单胞菌门相对丰度显著增加,绿弯菌门、不动杆菌门、拟杆菌门等显著降低.LEfSe结果表明,黄精轮作后三七根腐病病原菌镰刀菌属、Ilyonectria等真菌显著降低;芽孢杆菌属、芽单胞菌属、溶杆菌属、类芽胞杆菌属等重要细菌属显著增加.根际土壤微生物真菌主要以腐生、病理以及混合营养模式为主,经黄精轮作处理后,植物病原物真菌丰度下降,致病菌的占比有所下调,与病害有关的微生物下降,同时黄精轮作后细菌L-缬氨酸生物合成途径、L-异亮氨酸生物合成1(来自苏氨酸)途径、L-异亮氨酸生物合成Ⅱ、有氧呼吸Ⅰ(细胞色素C)途径显著降低.利用黄精轮作能够改善土壤微生物,对缓解三七连作障碍具有一定潜力.
Analysis of the potential of Polygonatum sibiricum rotation to improve soil microbi-al community and alleviate continuous cropping obstacle of Panax notoginseng
In order to explore the influence of Polygonatum sibiricum rotation on the soil microbial community of Panax notoginseng,evaluate the restoration ability of crop rotation and natural restoration.The influence of Polygonatum sibiricum rotation on the microbial structure and microflora change of soil continuous cropping with Panax notoginseng was studied.The results showed that seedling rate was 88.33%,significantly higher than the incidence rate of fallow treatment,which was less than 20%,but the difference was not significant.The diversity of soil fungi and bacteria was significantly reduced(P<0.01).The results showed that crop rotation could signi-ficantly affect the β diversity of soil fungi and bacteria in Panax notoginseng(P<0.001),and could significantly increase the relative abundance of Ascomycota and reduce the relative abundance of Basidiomycota and Triguta.Firmicutes,Budlesomonas,Chloritans,Acinetobacter,Bacteroidetes,etc.LEfSe results showed that after the rotation of the pathogens,Fusarium,Ilyonectria,and other fungi.Rhizosphere soil microbial fungi mainly sa-prophytic,pathological and mixed nutrition mode,after the rotation treatment,plant pathogen fungi abundance,pathogenic bacteria of decline,associated with the disease,at the same time after rotation bacterial L-valine bio-synthesis pathway,L-isoleucine biosynthesis Ⅰ(from threonine)pathway,L-isoleucine biosynthesis Ⅱ,Aerobic respiration Ⅰ(cytochrome C)pathway is significantly lower.The rotation of Polygonatum sibiricum can improve soil microorganisms and it's potential to alleviate the obstacles of Panax notoginseng.

Panax notoginsengPolygonatum sibiricumcrop rotationsoil microorganismcontinuous obstacles

陈子涵、何涛、施本义、李科、蔡红、何霞红、朱有勇、赵宇

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云南农业大学农业生物多样性应用技术国家工程研究中心,农业生物多样性与病虫害控制教育部重点实验室,昆明 650201

澜沧良宝生物科技有限公司,普洱 665000

西南林业大学园林园艺学院,昆明 650224

三七 黄精 轮作 土壤微生物 连作障碍

国家重点研发计划云南省科技重大专项&&

2021YFD1000204202002AE90140202002AE320004

2024

植物病理学报
中国植物病理学会

植物病理学报

CSTPCD北大核心
影响因子:0.865
ISSN:0412-0914
年,卷(期):2024.54(4)